34
{mso-list-id:734164761;
mso-list-type:simple;
mso-list-template-ids:67698703;}
@list l134:level1
{mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;}
@list l135
{mso-list-id:743063349;
mso-list-type:simple;
mso-list-template-ids:67698703;}
@list l135:level1
{mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;}
@list l136
{mso-list-id:753357657;
mso-list-template-ids:815453410;}
@list l136:level1
{mso-level-tab-stop:.75in;
mso-level-number-position:left;
margin-left:.75in;
text-indent:-.5in;}
@list l137
{mso-list-id:754328021;
mso-list-type:simple;
mso-list-template-ids:624745472;}
@list l137:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l138
{mso-list-id:759914661;
mso-list-type:simple;
mso-list-template-ids:67698689;}
@list l138:level1
{mso-level-number-format:bullet;
mso-level-text:\F0B7;
mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;
font-family:Symbol;}
@list l139
{mso-list-id:787050170;
mso-list-template-ids:1332641892;}
@list l139:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.25in;}
@list l140
{mso-list-id:803424383;
mso-list-type:simple;
mso-list-template-ids:67698689;}
@list l140:level1
{mso-level-number-format:bullet;
mso-level-text:\F0B7;
mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;
font-family:Symbol;}
@list l141
{mso-list-id:810907645;
mso-list-type:simple;
mso-list-template-ids:255883122;}
@list l141:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;
mso-ansi-font-weight:bold;
mso-ansi-font-style:italic;}
@list l142
{mso-list-id:813058984;
mso-list-type:simple;
mso-list-template-ids:67698689;}
@list l142:level1
{mso-level-number-format:bullet;
mso-level-text:\F0B7;
mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;
font-family:Symbol;}
@list l143
{mso-list-id:819808396;
mso-list-template-ids:534172858;}
@list l143:level1
{mso-level-start-at:13;
mso-level-tab-stop:.75in;
mso-level-number-position:left;
margin-left:.75in;
text-indent:-.5in;}
@list l144
{mso-list-id:821044076;
mso-list-type:simple;
mso-list-template-ids:575717982;}
@list l144:level1
{mso-level-start-at:52;
mso-level-tab-stop:42.0pt;
mso-level-number-position:left;
margin-left:42.0pt;
text-indent:-42.0pt;}
@list l145
{mso-list-id:825632662;
mso-list-template-ids:-652812694;}
@list l145:level1
{mso-level-tab-stop:.75in;
mso-level-number-position:left;
margin-left:.75in;
text-indent:-.5in;}
@list l146
{mso-list-id:840387241;
mso-list-type:simple;
mso-list-template-ids:-520306082;}
@list l146:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l147
{mso-list-id:847208067;
mso-list-type:simple;
mso-list-template-ids:429567930;}
@list l147:level1
{mso-level-number-format:roman-lower;
mso-level-text:"\(%1\)";
mso-level-tab-stop:1.0in;
mso-level-number-position:left;
margin-left:1.0in;
text-indent:-.5in;}
@list l148
{mso-list-id:849681074;
mso-list-type:simple;
mso-list-template-ids:-999106500;}
@list l148:level1
{mso-level-tab-stop:1.0in;
mso-level-number-position:left;
margin-left:1.0in;
text-indent:-.5in;
mso-ansi-font-weight:normal;}
@list l149
{mso-list-id:850027778;
mso-list-type:simple;
mso-list-template-ids:67698689;}
@list l149:level1
{mso-level-number-format:bullet;
mso-level-text:\F0B7;
mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;
font-family:Symbol;}
@list l150
{mso-list-id:852033917;
mso-list-type:hybrid;
mso-list-template-ids:142004698 67698703 67698713 67698715 67698703 67698713 67698715 67698703 67698713 67698715;}
@list l150:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.25in;}
@list l151
{mso-list-id:853304500;
mso-list-type:hybrid;
mso-list-template-ids:990676156;}
@list l151:level1
{mso-level-number-format:bullet;
mso-level-text:\F0B7;
mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.25in;
mso-ansi-font-size:10.0pt;
font-family:Symbol;}
@list l152
{mso-list-id:856621579;
mso-list-type:simple;
mso-list-template-ids:107015108;}
@list l152:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l153
{mso-list-id:857818960;
mso-list-type:hybrid;
mso-list-template-ids:-404600030 1075096902 -515454710 67698715 67698703 67698713 67698715 67698703 67698713 67698715;}
@list l153:level1
{mso-level-tab-stop:.75in;
mso-level-number-position:left;
margin-left:.75in;
text-indent:-.5in;}
@list l153:level2
{mso-level-start-at:2;
mso-level-number-format:alpha-upper;
mso-level-tab-stop:1.0in;
mso-level-number-position:left;
text-indent:-.25in;}
@list l154
{mso-list-id:862521724;
mso-list-type:simple;
mso-list-template-ids:-143104456;}
@list l154:level1
{mso-level-start-at:2;
mso-level-text:%1;
mso-level-tab-stop:24.0pt;
mso-level-number-position:left;
margin-left:24.0pt;
text-indent:-24.0pt;}
@list l155
{mso-list-id:866531301;
mso-list-type:simple;
mso-list-template-ids:67698689;}
@list l155:level1
{mso-level-number-format:bullet;
mso-level-text:\F0B7;
mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;
font-family:Symbol;}
@list l156
{mso-list-id:876890245;
mso-list-type:hybrid;
mso-list-template-ids:-1520916498 67698703 67698713 67698715 67698703 67698713 67698715 67698703 67698713 67698715;}
@list l156:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.25in;}
@list l157
{mso-list-id:876892237;
mso-list-type:simple;
mso-list-template-ids:-1600093828;}
@list l157:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l158
{mso-list-id:876964808;
mso-list-type:simple;
mso-list-template-ids:1883770800;}
@list l158:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l159
{mso-list-id:878929697;
mso-list-type:simple;
mso-list-template-ids:1541950532;}
@list l159:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.25in;}
@list l160
{mso-list-id:880678004;
mso-list-type:simple;
mso-list-template-ids:-1793039650;}
@list l160:level1
{mso-level-start-at:14;
mso-level-tab-stop:20.25pt;
mso-level-number-position:left;
margin-left:20.25pt;
text-indent:-20.25pt;}
@list l161
{mso-list-id:894389145;
mso-list-type:simple;
mso-list-template-ids:-1271920838;}
@list l161:level1
{mso-level-start-at:7;
mso-level-text:%1;
mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;}
@list l162
{mso-list-id:897133463;
mso-list-type:simple;
mso-list-template-ids:-2098060564;}
@list l162:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l163
{mso-list-id:906040216;
mso-list-type:simple;
mso-list-template-ids:67698703;}
@list l163:level1
{mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;}
@list l164
{mso-list-id:912004112;
mso-list-type:simple;
mso-list-template-ids:67698689;}
@list l164:level1
{mso-level-number-format:bullet;
mso-level-text:\F0B7;
mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;
font-family:Symbol;}
@list l165
{mso-list-id:917977451;
mso-list-type:simple;
mso-list-template-ids:67698703;}
@list l165:level1
{mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;}
@list l166
{mso-list-id:918708477;
mso-list-type:simple;
mso-list-template-ids:777307304;}
@list l166:level1
{mso-level-start-at:8;
mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l167
{mso-list-id:922681777;
mso-list-type:hybrid;
mso-list-template-ids:-615594308 67698703 67698713 67698715 67698703 67698713 67698715 67698703 67698713 67698715;}
@list l167:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.25in;}
@list l168
{mso-list-id:929050232;
mso-list-type:simple;
mso-list-template-ids:-929253372;}
@list l168:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;
mso-ansi-font-weight:bold;}
@list l169
{mso-list-id:936329016;
mso-list-template-ids:330344114;}
@list l169:level1
{mso-level-tab-stop:.75in;
mso-level-number-position:left;
margin-left:.75in;
text-indent:-.5in;}
@list l170
{mso-list-id:945964690;
mso-list-type:simple;
mso-list-template-ids:67698689;}
@list l170:level1
{mso-level-number-format:bullet;
mso-level-text:\F0B7;
mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;
font-family:Symbol;}
@list l171
{mso-list-id:950550308;
mso-list-template-ids:-667545940;}
@list l171:level1
{mso-level-tab-stop:.75in;
mso-level-number-position:left;
margin-left:.75in;
text-indent:-.5in;}
@list l172
{mso-list-id:950556163;
mso-list-type:simple;
mso-list-template-ids:-639568308;}
@list l172:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;
mso-ansi-font-weight:bold;}
@list l173
{mso-list-id:956834060;
mso-list-type:hybrid;
mso-list-template-ids:815987634;}
@list l173:level1
{mso-level-tab-stop:.75in;
mso-level-number-position:left;
margin-left:.75in;
text-indent:-.5in;}
@list l174
{mso-list-id:962350297;
mso-list-type:simple;
mso-list-template-ids:34004356;}
@list l174:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l175
{mso-list-id:969942492;
mso-list-type:simple;
mso-list-template-ids:-988377902;}
@list l175:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l176
{mso-list-id:970211566;
mso-list-type:hybrid;
mso-list-template-ids:203315626 1925235280 67698713 67698715 67698703 67698713 67698715 67698703 67698713 67698715;}
@list l176:level1
{mso-level-tab-stop:57.0pt;
mso-level-number-position:left;
margin-left:57.0pt;
text-indent:-39.0pt;}
@list l177
{mso-list-id:977077432;
mso-list-type:simple;
mso-list-template-ids:-1905650428;}
@list l177:level1
{mso-level-start-at:4;
mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l178
{mso-list-id:977877290;
mso-list-type:simple;
mso-list-template-ids:-1601941730;}
@list l178:level1
{mso-level-number-format:alpha-lower;
mso-level-text:"\(%1\)";
mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l179
{mso-list-id:978723338;
mso-list-type:simple;
mso-list-template-ids:8808728;}
@list l179:level1
{mso-level-start-at:15;
mso-level-text:%1;
mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;
mso-ansi-font-weight:bold;}
@list l180
{mso-list-id:981740609;
mso-list-type:hybrid;
mso-list-template-ids:467560550;}
@list l180:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.25in;}
@list l181
{mso-list-id:983319648;
mso-list-type:simple;
mso-list-template-ids:67698689;}
@list l181:level1
{mso-level-number-format:bullet;
mso-level-text:\F0B7;
mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;
font-family:Symbol;}
@list l182
{mso-list-id:995451605;
mso-list-type:simple;
mso-list-template-ids:-1037792332;}
@list l182:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l183
{mso-list-id:995646417;
mso-list-type:simple;
mso-list-template-ids:67698703;}
@list l183:level1
{mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;}
@list l184
{mso-list-id:1009721535;
mso-list-type:simple;
mso-list-template-ids:-269456364;}
@list l184:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l185
{mso-list-id:1009795389;
mso-list-type:simple;
mso-list-template-ids:67698703;}
@list l185:level1
{mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;}
@list l186
{mso-list-id:1014846828;
mso-list-template-ids:-671316730;}
@list l186:level1
{mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;}
@list l186:level2
{mso-level-legal-format:yes;
mso-level-text:"%1\.%2";
mso-level-tab-stop:51.75pt;
mso-level-number-position:left;
margin-left:51.75pt;
text-indent:-36.75pt;}
@list l186:level3
{mso-level-legal-format:yes;
mso-level-text:"%1\.%2\.%3";
mso-level-tab-stop:66.75pt;
mso-level-number-position:left;
margin-left:66.75pt;
text-indent:-36.75pt;}
@list l186:level4
{mso-level-legal-format:yes;
mso-level-text:"%1\.%2\.%3\.%4";
mso-level-tab-stop:99.0pt;
mso-level-number-position:left;
margin-left:99.0pt;
text-indent:-.75in;}
@list l186:level5
{mso-level-legal-format:yes;
mso-level-text:"%1\.%2\.%3\.%4\.%5";
mso-level-tab-stop:132.0pt;
mso-level-number-position:left;
margin-left:132.0pt;
text-indent:-1.0in;}
@list l186:level6
{mso-level-legal-format:yes;
mso-level-text:"%1\.%2\.%3\.%4\.%5\.%6";
mso-level-tab-stop:147.0pt;
mso-level-number-position:left;
margin-left:147.0pt;
text-indent:-1.0in;}
@list l186:level7
{mso-level-legal-format:yes;
mso-level-text:"%1\.%2\.%3\.%4\.%5\.%6\.%7";
mso-level-tab-stop:2.5in;
mso-level-number-position:left;
margin-left:2.5in;
text-indent:-1.25in;}
@list l186:level8
{mso-level-legal-format:yes;
mso-level-text:"%1\.%2\.%3\.%4\.%5\.%6\.%7\.%8";
mso-level-tab-stop:213.0pt;
mso-level-number-position:left;
margin-left:213.0pt;
text-indent:-1.5in;}
@list l186:level9
{mso-level-legal-format:yes;
mso-level-text:"%1\.%2\.%3\.%4\.%5\.%6\.%7\.%8\.%9";
mso-level-tab-stop:228.0pt;
mso-level-number-position:left;
margin-left:228.0pt;
text-indent:-1.5in;}
@list l187
{mso-list-id:1018046924;
mso-list-type:simple;
mso-list-template-ids:-988377902;}
@list l187:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l188
{mso-list-id:1026756633;
mso-list-type:simple;
mso-list-template-ids:-1736823296;}
@list l188:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l189
{mso-list-id:1028943259;
mso-list-type:simple;
mso-list-template-ids:67698703;}
@list l189:level1
{mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;
mso-ansi-font-weight:normal;
text-decoration:none;
text-underline:none;}
@list l190
{mso-list-id:1038622990;
mso-list-type:simple;
mso-list-template-ids:67698689;}
@list l190:level1
{mso-level-number-format:bullet;
mso-level-text:\F0B7;
mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;
font-family:Symbol;}
@list l191
{mso-list-id:1056584083;
mso-list-type:simple;
mso-list-template-ids:67698703;}
@list l191:level1
{mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;}
@list l192
{mso-list-id:1059666880;
mso-list-template-ids:-11910530;}
@list l192:level1
{mso-level-tab-stop:.75in;
mso-level-number-position:left;
margin-left:.75in;
text-indent:-.5in;}
@list l193
{mso-list-id:1060135529;
mso-list-type:simple;
mso-list-template-ids:1569863614;}
@list l193:level1
{mso-level-start-at:25;
mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l194
{mso-list-id:1068307014;
mso-list-type:simple;
mso-list-template-ids:67698689;}
@list l194:level1
{mso-level-number-format:bullet;
mso-level-text:\F0B7;
mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;
font-family:Symbol;}
@list l195
{mso-list-id:1071806420;
mso-list-type:hybrid;
mso-list-template-ids:-2138155924;}
@list l195:level1
{mso-level-tab-stop:.75in;
mso-level-number-position:left;
margin-left:.75in;
text-indent:-.5in;}
@list l196
{mso-list-id:1071999719;
mso-list-type:simple;
mso-list-template-ids:-533018240;}
@list l196:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l197
{mso-list-id:1072698202;
mso-list-type:simple;
mso-list-template-ids:67698689;}
@list l197:level1
{mso-level-number-format:bullet;
mso-level-text:\F0B7;
mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;
font-family:Symbol;}
@list l198
{mso-list-id:1076702636;
mso-list-type:simple;
mso-list-template-ids:67698689;}
@list l198:level1
{mso-level-number-format:bullet;
mso-level-text:\F0B7;
mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;
font-family:Symbol;}
@list l199
{mso-list-id:1078866897;
mso-list-type:simple;
mso-list-template-ids:123218198;}
@list l199:level1
{mso-level-start-at:18;
mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;
mso-ansi-font-style:normal;}
@list l200
{mso-list-id:1080952746;
mso-list-type:simple;
mso-list-template-ids:67698689;}
@list l200:level1
{mso-level-number-format:bullet;
mso-level-text:\F0B7;
mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;
font-family:Symbol;}
@list l201
{mso-list-id:1082727137;
mso-list-type:simple;
mso-list-template-ids:67698703;}
@list l201:level1
{mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;}
@list l202
{mso-list-id:1083332499;
mso-list-type:hybrid;
mso-list-template-ids:-603714038;}
@list l202:level1
{mso-level-number-format:bullet;
mso-level-text:\F0B7;
mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.25in;
mso-ansi-font-size:10.0pt;
font-family:Symbol;}
@list l203
{mso-list-id:1092821270;
mso-list-type:simple;
mso-list-template-ids:27063444;}
@list l203:level1
{mso-level-number-format:roman-lower;
mso-level-text:"%1\)";
mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l204
{mso-list-id:1093747714;
mso-list-type:simple;
mso-list-template-ids:-1551211606;}
@list l204:level1
{mso-level-start-at:12;
mso-level-tab-stop:.75in;
mso-level-number-position:left;
margin-left:.75in;
text-indent:-.25in;}
@list l205
{mso-list-id:1096513490;
mso-list-type:simple;
mso-list-template-ids:67698703;}
@list l205:level1
{mso-level-start-at:19;
mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;}
@list l206
{mso-list-id:1096906896;
mso-list-type:simple;
mso-list-template-ids:349469082;}
@list l206:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l207
{mso-list-id:1116363532;
mso-list-type:simple;
mso-list-template-ids:1473809800;}
@list l207:level1
{mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;
mso-ansi-font-size:14.0pt;
mso-ansi-font-weight:bold;}
@list l208
{mso-list-id:1119371622;
mso-list-type:simple;
mso-list-template-ids:67698693;}
@list l208:level1
{mso-level-number-format:bullet;
mso-level-text:\F0A7;
mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;
font-family:Wingdings;}
@list l209
{mso-list-id:1124426320;
mso-list-template-ids:-671316730;}
@list l209:level1
{mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;}
@list l209:level2
{mso-level-legal-format:yes;
mso-level-text:"%1\.%2";
mso-level-tab-stop:51.75pt;
mso-level-number-position:left;
margin-left:51.75pt;
text-indent:-36.75pt;}
@list l209:level3
{mso-level-legal-format:yes;
mso-level-text:"%1\.%2\.%3";
mso-level-tab-stop:66.75pt;
mso-level-number-position:left;
margin-left:66.75pt;
text-indent:-36.75pt;}
@list l209:level4
{mso-level-legal-format:yes;
mso-level-text:"%1\.%2\.%3\.%4";
mso-level-tab-stop:99.0pt;
mso-level-number-position:left;
margin-left:99.0pt;
text-indent:-.75in;}
@list l209:level5
{mso-level-legal-format:yes;
mso-level-text:"%1\.%2\.%3\.%4\.%5";
mso-level-tab-stop:132.0pt;
mso-level-number-position:left;
margin-left:132.0pt;
text-indent:-1.0in;}
@list l209:level6
{mso-level-legal-format:yes;
mso-level-text:"%1\.%2\.%3\.%4\.%5\.%6";
mso-level-tab-stop:147.0pt;
mso-level-number-position:left;
margin-left:147.0pt;
text-indent:-1.0in;}
@list l209:level7
{mso-level-legal-format:yes;
mso-level-text:"%1\.%2\.%3\.%4\.%5\.%6\.%7";
mso-level-tab-stop:2.5in;
mso-level-number-position:left;
margin-left:2.5in;
text-indent:-1.25in;}
@list l209:level8
{mso-level-legal-format:yes;
mso-level-text:"%1\.%2\.%3\.%4\.%5\.%6\.%7\.%8";
mso-level-tab-stop:213.0pt;
mso-level-number-position:left;
margin-left:213.0pt;
text-indent:-1.5in;}
@list l209:level9
{mso-level-legal-format:yes;
mso-level-text:"%1\.%2\.%3\.%4\.%5\.%6\.%7\.%8\.%9";
mso-level-tab-stop:228.0pt;
mso-level-number-position:left;
margin-left:228.0pt;
text-indent:-1.5in;}
@list l210
{mso-list-id:1140726884;
mso-list-type:simple;
mso-list-template-ids:-1;}
@list l210:level1
{mso-level-number-format:bullet;
mso-level-text:\F0B7;
mso-level-tab-stop:none;
mso-level-number-position:left;
mso-level-legacy:yes;
mso-level-legacy-indent:.25in;
mso-level-legacy-space:0in;
margin-left:1.75in;
text-indent:-.25in;
font-family:Symbol;}
@list l211
{mso-list-id:1162626027;
mso-list-type:simple;
mso-list-template-ids:67698703;}
@list l211:level1
{mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;}
@list l212
{mso-list-id:1162697536;
mso-list-type:simple;
mso-list-template-ids:67698703;}
@list l212:level1
{mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;}
@list l213
{mso-list-id:1169753320;
mso-list-type:hybrid;
mso-list-template-ids:-393423524;}
@list l213:level1
{mso-level-tab-stop:.75in;
mso-level-number-position:left;
margin-left:.75in;
text-indent:-.5in;}
@list l214
{mso-list-id:1172374951;
mso-list-type:simple;
mso-list-template-ids:147868832;}
@list l214:level1
{mso-level-number-format:alpha-lower;
mso-level-text:"\(%1\)";
mso-level-tab-stop:1.0in;
mso-level-number-position:left;
margin-left:1.0in;
text-indent:-.5in;}
@list l215
{mso-list-id:1172993071;
mso-list-type:hybrid;
mso-list-template-ids:-1661537936;}
@list l215:level1
{mso-level-tab-stop:.75in;
mso-level-number-position:left;
margin-left:.75in;
text-indent:-.5in;}
@list l216
{mso-list-id:1179588844;
mso-list-type:simple;
mso-list-template-ids:-988377902;}
@list l216:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l217
{mso-list-id:1179614547;
mso-list-type:hybrid;
mso-list-template-ids:-422253790;}
@list l217:level1
{mso-level-tab-stop:.75in;
mso-level-number-position:left;
margin-left:.75in;
text-indent:-.5in;}
@list l218
{mso-list-id:1181893398;
mso-list-type:simple;
mso-list-template-ids:67698689;}
@list l218:level1
{mso-level-number-format:bullet;
mso-level-text:\F0B7;
mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;
font-family:Symbol;}
@list l219
{mso-list-id:1183399687;
mso-list-type:simple;
mso-list-template-ids:67698689;}
@list l219:level1
{mso-level-number-format:bullet;
mso-level-text:\F0B7;
mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;
font-family:Symbol;}
@list l220
{mso-list-id:1186284458;
mso-list-type:simple;
mso-list-template-ids:-988377902;}
@list l220:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l221
{mso-list-id:1190408676;
mso-list-type:simple;
mso-list-template-ids:-1271920838;}
@list l221:level1
{mso-level-start-at:7;
mso-level-text:%1;
mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;}
@list l222
{mso-list-id:1191410489;
mso-list-type:simple;
mso-list-template-ids:67698689;}
@list l222:level1
{mso-level-number-format:bullet;
mso-level-text:\F0B7;
mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;
font-family:Symbol;}
@list l223
{mso-list-id:1192693373;
mso-list-type:hybrid;
mso-list-template-ids:2085807576;}
@list l223:level1
{mso-level-tab-stop:.75in;
mso-level-number-position:left;
margin-left:.75in;
text-indent:-.5in;}
@list l224
{mso-list-id:1194464286;
mso-list-type:simple;
mso-list-template-ids:-1271920838;}
@list l224:level1
{mso-level-start-at:7;
mso-level-text:%1;
mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;}
@list l225
{mso-list-id:1204370208;
mso-list-type:simple;
mso-list-template-ids:67698703;}
@list l225:level1
{mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;}
@list l226
{mso-list-id:1204944990;
mso-list-type:simple;
mso-list-template-ids:67698689;}
@list l226:level1
{mso-level-number-format:bullet;
mso-level-text:\F0B7;
mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;
font-family:Symbol;}
@list l227
{mso-list-id:1205560404;
mso-list-type:hybrid;
mso-list-template-ids:-2039172812 1075096902 67698713 67698715 67698703 67698713 67698715 67698703 67698713 67698715;}
@list l227:level1
{mso-level-tab-stop:.75in;
mso-level-number-position:left;
margin-left:.75in;
text-indent:-.5in;}
@list l228
{mso-list-id:1206529727;
mso-list-type:simple;
mso-list-template-ids:-1;}
@list l228:level1
{mso-level-number-format:bullet;
mso-level-text:\F0B7;
mso-level-tab-stop:none;
mso-level-number-position:left;
mso-level-legacy:yes;
mso-level-legacy-indent:.25in;
mso-level-legacy-space:0in;
margin-left:1.75in;
text-indent:-.25in;
font-family:Symbol;}
@list l229
{mso-list-id:1217429497;
mso-list-type:simple;
mso-list-template-ids:1894408896;}
@list l229:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;
mso-ansi-font-weight:bold;}
@list l230
{mso-list-id:1236668094;
mso-list-type:simple;
mso-list-template-ids:67698703;}
@list l230:level1
{mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;}
@list l231
{mso-list-id:1248073923;
mso-list-type:simple;
mso-list-template-ids:67698689;}
@list l231:level1
{mso-level-number-format:bullet;
mso-level-text:\F0B7;
mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;
font-family:Symbol;}
@list l232
{mso-list-id:1249998576;
mso-list-type:simple;
mso-list-template-ids:34004356;}
@list l232:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l233
{mso-list-id:1257058475;
mso-list-type:simple;
mso-list-template-ids:67698703;}
@list l233:level1
{mso-level-start-at:8;
mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;}
@list l234
{mso-list-id:1260526160;
mso-list-template-ids:330344114;}
@list l234:level1
{mso-level-tab-stop:.75in;
mso-level-number-position:left;
margin-left:.75in;
text-indent:-.5in;}
@list l235
{mso-list-id:1265725613;
mso-list-type:simple;
mso-list-template-ids:-515834476;}
@list l235:level1
{mso-level-start-at:12;
mso-level-text:%1;
mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;}
@list l236
{mso-list-id:1268850565;
mso-list-type:simple;
mso-list-template-ids:-988377902;}
@list l236:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l237
{mso-list-id:1269697438;
mso-list-type:simple;
mso-list-template-ids:67698703;}
@list l237:level1
{mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;}
@list l238
{mso-list-id:1270889067;
mso-list-type:simple;
mso-list-template-ids:67698689;}
@list l238:level1
{mso-level-number-format:bullet;
mso-level-text:\F0B7;
mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;
font-family:Symbol;}
@list l239
{mso-list-id:1274097543;
mso-list-type:simple;
mso-list-template-ids:380299058;}
@list l239:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l240
{mso-list-id:1277712578;
mso-list-type:hybrid;
mso-list-template-ids:-1276708424;}
@list l240:level1
{mso-level-tab-stop:.75in;
mso-level-number-position:left;
margin-left:.75in;
text-indent:-.5in;}
@list l241
{mso-list-id:1278102572;
mso-list-type:simple;
mso-list-template-ids:67698689;}
@list l241:level1
{mso-level-number-format:bullet;
mso-level-text:\F0B7;
mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;
font-family:Symbol;}
@list l242
{mso-list-id:1280605889;
mso-list-type:simple;
mso-list-template-ids:67698689;}
@list l242:level1
{mso-level-number-format:bullet;
mso-level-text:\F0B7;
mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;
font-family:Symbol;}
@list l243
{mso-list-id:1285961995;
mso-list-type:simple;
mso-list-template-ids:717253498;}
@list l243:level1
{mso-level-number-format:alpha-lower;
mso-level-text:"\(%1\)";
mso-level-tab-stop:1.0in;
mso-level-number-position:left;
margin-left:1.0in;
text-indent:-.5in;}
@list l244
{mso-list-id:1286422757;
mso-list-type:simple;
mso-list-template-ids:67698689;}
@list l244:level1
{mso-level-number-format:bullet;
mso-level-text:\F0B7;
mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;
font-family:Symbol;}
@list l245
{mso-list-id:1291667347;
mso-list-type:simple;
mso-list-template-ids:-289273672;}
@list l245:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l246
{mso-list-id:1296332739;
mso-list-type:simple;
mso-list-template-ids:67698689;}
@list l246:level1
{mso-level-number-format:bullet;
mso-level-text:\F0B7;
mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;
font-family:Symbol;}
@list l247
{mso-list-id:1302885896;
mso-list-type:simple;
mso-list-template-ids:-1054993028;}
@list l247:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l248
{mso-list-id:1305088209;
mso-list-type:simple;
mso-list-template-ids:-2073164976;}
@list l248:level1
{mso-level-text:%1;
mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;}
@list l249
{mso-list-id:1306548804;
mso-list-type:simple;
mso-list-template-ids:67698689;}
@list l249:level1
{mso-level-number-format:bullet;
mso-level-text:\F0B7;
mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;
font-family:Symbol;}
@list l250
{mso-list-id:1309554911;
mso-list-type:simple;
mso-list-template-ids:-393577160;}
@list l250:level1
{mso-level-start-at:5;
mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l251
{mso-list-id:1313407031;
mso-list-template-ids:-652812694;}
@list l251:level1
{mso-level-tab-stop:.75in;
mso-level-number-position:left;
margin-left:.75in;
text-indent:-.5in;}
@list l252
{mso-list-id:1313410240;
mso-list-template-ids:-652812694;}
@list l252:level1
{mso-level-tab-stop:.75in;
mso-level-number-position:left;
margin-left:.75in;
text-indent:-.5in;}
@list l253
{mso-list-id:1316564314;
mso-list-type:simple;
mso-list-template-ids:67698703;}
@list l253:level1
{mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;}
@list l254
{mso-list-id:1321613131;
mso-list-type:simple;
mso-list-template-ids:-651423728;}
@list l254:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l255
{mso-list-id:1330674949;
mso-list-type:simple;
mso-list-template-ids:395577000;}
@list l255:level1
{mso-level-start-at:9;
mso-level-text:%1;
mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;}
@list l256
{mso-list-id:1337266491;
mso-list-template-ids:1847370346;}
@list l256:level1
{mso-level-tab-stop:.75in;
mso-level-number-position:left;
margin-left:.75in;
text-indent:-.5in;}
@list l257
{mso-list-id:1350138631;
mso-list-type:simple;
mso-list-template-ids:67698689;}
@list l257:level1
{mso-level-number-format:bullet;
mso-level-text:\F0B7;
mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;
font-family:Symbol;}
@list l258
{mso-list-id:1356347729;
mso-list-type:simple;
mso-list-template-ids:233752124;}
@list l258:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;
mso-ansi-font-weight:normal;}
@list l259
{mso-list-id:1356469122;
mso-list-type:simple;
mso-list-template-ids:1008113972;}
@list l259:level1
{mso-level-number-format:roman-lower;
mso-level-text:"\(%1\)";
mso-level-tab-stop:1.0in;
mso-level-number-position:left;
margin-left:1.0in;
text-indent:-.5in;}
@list l260
{mso-list-id:1364867037;
mso-list-type:simple;
mso-list-template-ids:67698703;}
@list l260:level1
{mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;}
@list l261
{mso-list-id:1375421748;
mso-list-type:simple;
mso-list-template-ids:67698689;}
@list l261:level1
{mso-level-number-format:bullet;
mso-level-text:\F0B7;
mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;
font-family:Symbol;}
@list l262
{mso-list-id:1377898999;
mso-list-type:simple;
mso-list-template-ids:-243791040;}
@list l262:level1
{mso-level-start-at:3;
mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;}
@list l263
{mso-list-id:1379861557;
mso-list-type:simple;
mso-list-template-ids:67698689;}
@list l263:level1
{mso-level-number-format:bullet;
mso-level-text:\F0B7;
mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;
font-family:Symbol;}
@list l264
{mso-list-id:1387870917;
mso-list-type:simple;
mso-list-template-ids:-1269379738;}
@list l264:level1
{mso-level-start-at:28;
mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;
text-decoration:none;
text-underline:none;}
@list l265
{mso-list-id:1390298490;
mso-list-type:simple;
mso-list-template-ids:67698699;}
@list l265:level1
{mso-level-number-format:bullet;
mso-level-text:\F0D8;
mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;
font-family:Wingdings;}
@list l266
{mso-list-id:1396125424;
mso-list-type:simple;
mso-list-template-ids:-775244164;}
@list l266:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l267
{mso-list-id:1402289951;
mso-list-type:hybrid;
mso-list-template-ids:-388090352;}
@list l267:level1
{mso-level-tab-stop:.75in;
mso-level-number-position:left;
margin-left:.75in;
text-indent:-.5in;}
@list l268
{mso-list-id:1406805319;
mso-list-type:simple;
mso-list-template-ids:67698703;}
@list l268:level1
{mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;}
@list l269
{mso-list-id:1411584996;
mso-list-type:simple;
mso-list-template-ids:67698703;}
@list l269:level1
{mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;}
@list l270
{mso-list-id:1416174296;
mso-list-type:simple;
mso-list-template-ids:67698703;}
@list l270:level1
{mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;}
@list l271
{mso-list-id:1430193924;
mso-list-type:simple;
mso-list-template-ids:-302216654;}
@list l271:level1
{mso-level-start-at:10;
mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l272
{mso-list-id:1431005827;
mso-list-type:simple;
mso-list-template-ids:-524230512;}
@list l272:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l273
{mso-list-id:1437868621;
mso-list-type:simple;
mso-list-template-ids:-1;}
@list l273:level1
{mso-level-number-format:bullet;
mso-level-text:\F0B7;
mso-level-tab-stop:none;
mso-level-number-position:left;
mso-level-legacy:yes;
mso-level-legacy-indent:.25in;
mso-level-legacy-space:0in;
margin-left:1.75in;
text-indent:-.25in;
font-family:Symbol;}
@list l274
{mso-list-id:1438594824;
mso-list-type:simple;
mso-list-template-ids:67698689;}
@list l274:level1
{mso-level-number-format:bullet;
mso-level-text:\F0B7;
mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;
font-family:Symbol;}
@list l275
{mso-list-id:1441216739;
mso-list-type:simple;
mso-list-template-ids:67698703;}
@list l275:level1
{mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;}
@list l276
{mso-list-id:1442459691;
mso-list-type:simple;
mso-list-template-ids:1417595738;}
@list l276:level1
{mso-level-start-at:5;
mso-level-number-format:alpha-lower;
mso-level-text:"\(%1\)";
mso-level-tab-stop:.75in;
mso-level-number-position:left;
margin-left:.75in;
text-indent:-.25in;
mso-ansi-font-weight:bold;
mso-ansi-font-style:italic;}
@list l277
{mso-list-id:1443112369;
mso-list-type:hybrid;
mso-list-template-ids:1239593678 1075096902 67698713 67698715 67698703 67698713 67698715 67698703 67698713 67698715;}
@list l277:level1
{mso-level-tab-stop:.75in;
mso-level-number-position:left;
margin-left:.75in;
text-indent:-.5in;}
@list l278
{mso-list-id:1444299355;
mso-list-type:simple;
mso-list-template-ids:-639479278;}
@list l278:level1
{mso-level-start-at:13;
mso-level-text:%1;
mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;}
@list l279
{mso-list-id:1457335365;
mso-list-type:simple;
mso-list-template-ids:979815716;}
@list l279:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l280
{mso-list-id:1464544957;
mso-list-type:simple;
mso-list-template-ids:-1836056406;}
@list l280:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l281
{mso-list-id:1473206045;
mso-list-type:simple;
mso-list-template-ids:67698703;}
@list l281:level1
{mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;}
@list l282
{mso-list-id:1476946253;
mso-list-template-ids:330344114;}
@list l282:level1
{mso-level-tab-stop:.75in;
mso-level-number-position:left;
margin-left:.75in;
text-indent:-.5in;}
@list l283
{mso-list-id:1481114701;
mso-list-type:simple;
mso-list-template-ids:67698689;}
@list l283:level1
{mso-level-number-format:bullet;
mso-level-text:\F0B7;
mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;
font-family:Symbol;}
@list l284
{mso-list-id:1482574798;
mso-list-type:simple;
mso-list-template-ids:-1369286738;}
@list l284:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l285
{mso-list-id:1482580984;
mso-list-type:simple;
mso-list-template-ids:-910293726;}
@list l285:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l286
{mso-list-id:1485320583;
mso-list-template-ids:2113320888;}
@list l286:level1
{mso-level-start-at:7;
mso-level-number-format:roman-upper;
mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l286:level2
{mso-level-legal-format:yes;
mso-level-text:"%1\.%2";
mso-level-tab-stop:51.75pt;
mso-level-number-position:left;
margin-left:51.75pt;
text-indent:-36.75pt;}
@list l286:level3
{mso-level-legal-format:yes;
mso-level-text:"%1\.%2\.%3";
mso-level-tab-stop:66.75pt;
mso-level-number-position:left;
margin-left:66.75pt;
text-indent:-36.75pt;}
@list l286:level4
{mso-level-legal-format:yes;
mso-level-text:"%1\.%2\.%3\.%4";
mso-level-tab-stop:99.0pt;
mso-level-number-position:left;
margin-left:99.0pt;
text-indent:-.75in;}
@list l286:level5
{mso-level-legal-format:yes;
mso-level-text:"%1\.%2\.%3\.%4\.%5";
mso-level-tab-stop:132.0pt;
mso-level-number-position:left;
margin-left:132.0pt;
text-indent:-1.0in;}
@list l286:level6
{mso-level-legal-format:yes;
mso-level-text:"%1\.%2\.%3\.%4\.%5\.%6";
mso-level-tab-stop:147.0pt;
mso-level-number-position:left;
margin-left:147.0pt;
text-indent:-1.0in;}
@list l286:level7
{mso-level-legal-format:yes;
mso-level-text:"%1\.%2\.%3\.%4\.%5\.%6\.%7";
mso-level-tab-stop:2.5in;
mso-level-number-position:left;
margin-left:2.5in;
text-indent:-1.25in;}
@list l286:level8
{mso-level-legal-format:yes;
mso-level-text:"%1\.%2\.%3\.%4\.%5\.%6\.%7\.%8";
mso-level-tab-stop:213.0pt;
mso-level-number-position:left;
margin-left:213.0pt;
text-indent:-1.5in;}
@list l286:level9
{mso-level-legal-format:yes;
mso-level-text:"%1\.%2\.%3\.%4\.%5\.%6\.%7\.%8\.%9";
mso-level-tab-stop:228.0pt;
mso-level-number-position:left;
margin-left:228.0pt;
text-indent:-1.5in;}
@list l287
{mso-list-id:1485663469;
mso-list-type:simple;
mso-list-template-ids:278315402;}
@list l287:level1
{mso-level-start-at:5;
mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;
mso-ansi-font-size:14.0pt;
mso-ansi-font-weight:bold;}
@list l288
{mso-list-id:1486507309;
mso-list-type:simple;
mso-list-template-ids:67698703;}
@list l288:level1
{mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;}
@list l289
{mso-list-id:1491287051;
mso-list-type:simple;
mso-list-template-ids:67698703;}
@list l289:level1
{mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;}
@list l290
{mso-list-id:1494225399;
mso-list-type:simple;
mso-list-template-ids:67698703;}
@list l290:level1
{mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;}
@list l291
{mso-list-id:1500190312;
mso-list-type:simple;
mso-list-template-ids:1800436510;}
@list l291:level1
{mso-level-start-at:3;
mso-level-number-format:alpha-lower;
mso-level-text:"\(%1\)";
mso-level-tab-stop:1.0in;
mso-level-number-position:left;
margin-left:1.0in;
text-indent:-.5in;
mso-ansi-font-weight:bold;
mso-ansi-font-style:italic;}
@list l292
{mso-list-id:1503203224;
mso-list-type:simple;
mso-list-template-ids:-710933498;}
@list l292:level1
{mso-level-start-at:7;
mso-level-text:%1;
mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l293
{mso-list-id:1507867997;
mso-list-template-ids:-652812694;}
@list l293:level1
{mso-level-tab-stop:.75in;
mso-level-number-position:left;
margin-left:.75in;
text-indent:-.5in;}
@list l294
{mso-list-id:1510024407;
mso-list-type:hybrid;
mso-list-template-ids:42883026;}
@list l294:level1
{mso-level-number-format:bullet;
mso-level-text:\F0B7;
mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.25in;
mso-ansi-font-size:10.0pt;
font-family:Symbol;}
@list l295
{mso-list-id:1511870188;
mso-list-type:simple;
mso-list-template-ids:202538926;}
@list l295:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l296
{mso-list-id:1523860976;
mso-list-type:simple;
mso-list-template-ids:-1271920838;}
@list l296:level1
{mso-level-start-at:7;
mso-level-text:%1;
mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;}
@list l297
{mso-list-id:1528759492;
mso-list-type:simple;
mso-list-template-ids:-23537410;}
@list l297:level1
{mso-level-start-at:132;
mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l298
{mso-list-id:1529680634;
mso-list-type:simple;
mso-list-template-ids:67698689;}
@list l298:level1
{mso-level-number-format:bullet;
mso-level-text:\F0B7;
mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;
font-family:Symbol;}
@list l299
{mso-list-id:1529752906;
mso-list-type:simple;
mso-list-template-ids:1184019514;}
@list l299:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l300
{mso-list-id:1530142422;
mso-list-template-ids:-652812694;}
@list l300:level1
{mso-level-tab-stop:.75in;
mso-level-number-position:left;
margin-left:.75in;
text-indent:-.5in;}
@list l301
{mso-list-id:1534273175;
mso-list-type:simple;
mso-list-template-ids:-988377902;}
@list l301:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l302
{mso-list-id:1540899697;
mso-list-template-ids:945967486;}
@list l302:level1
{mso-level-start-at:2;
mso-level-tab-stop:.75in;
mso-level-number-position:left;
margin-left:.75in;
text-indent:-.5in;}
@list l303
{mso-list-id:1543515947;
mso-list-type:simple;
mso-list-template-ids:-1750418168;}
@list l303:level1
{mso-level-start-at:10;
mso-level-text:%1;
mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;}
@list l304
{mso-list-id:1550335982;
mso-list-type:hybrid;
mso-list-template-ids:-1868891868 1075096902 67698713 67698715 67698703 67698713 67698715 67698703 67698713 67698715;}
@list l304:level1
{mso-level-tab-stop:.75in;
mso-level-number-position:left;
margin-left:.75in;
text-indent:-.5in;}
@list l305
{mso-list-id:1560632531;
mso-list-type:hybrid;
mso-list-template-ids:1943813752;}
@list l305:level1
{mso-level-tab-stop:1.0in;
mso-level-number-position:left;
margin-left:1.0in;
text-indent:-.5in;}
@list l306
{mso-list-id:1581670578;
mso-list-type:simple;
mso-list-template-ids:67698689;}
@list l306:level1
{mso-level-number-format:bullet;
mso-level-text:\F0B7;
mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;
font-family:Symbol;}
@list l307
{mso-list-id:1586569989;
mso-list-type:simple;
mso-list-template-ids:1564614416;}
@list l307:level1
{mso-level-number-format:roman-lower;
mso-level-text:"%1\)";
mso-level-tab-stop:1.0in;
mso-level-number-position:left;
margin-left:1.0in;
text-indent:-.5in;}
@list l308
{mso-list-id:1586724800;
mso-list-type:simple;
mso-list-template-ids:-2098060564;}
@list l308:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l309
{mso-list-id:1588731966;
mso-list-type:simple;
mso-list-template-ids:1422842040;}
@list l309:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l310
{mso-list-id:1605770650;
mso-list-type:hybrid;
mso-list-template-ids:330344114;}
@list l310:level1
{mso-level-tab-stop:.75in;
mso-level-number-position:left;
margin-left:.75in;
text-indent:-.5in;}
@list l311
{mso-list-id:1607275170;
mso-list-type:simple;
mso-list-template-ids:67698703;}
@list l311:level1
{mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;}
@list l312
{mso-list-id:1619214888;
mso-list-type:simple;
mso-list-template-ids:1431177692;}
@list l312:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l313
{mso-list-id:1624337311;
mso-list-type:simple;
mso-list-template-ids:67698689;}
@list l313:level1
{mso-level-number-format:bullet;
mso-level-text:\F0B7;
mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;
font-family:Symbol;}
@list l314
{mso-list-id:1626355056;
mso-list-type:hybrid;
mso-list-template-ids:2034768602 1401953702 67698713 67698715 67698703 67698713 67698715 67698703 67698713 67698715;}
@list l314:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.25in;}
@list l315
{mso-list-id:1626934555;
mso-list-type:simple;
mso-list-template-ids:67698689;}
@list l315:level1
{mso-level-number-format:bullet;
mso-level-text:\F0B7;
mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;
font-family:Symbol;}
@list l316
{mso-list-id:1632514007;
mso-list-type:simple;
mso-list-template-ids:-289273672;}
@list l316:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l317
{mso-list-id:1638758050;
mso-list-type:simple;
mso-list-template-ids:67698703;}
@list l317:level1
{mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;}
@list l318
{mso-list-id:1645549829;
mso-list-type:simple;
mso-list-template-ids:641872944;}
@list l318:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l319
{mso-list-id:1654531442;
mso-list-type:hybrid;
mso-list-template-ids:1644954968;}
@list l319:level1
{mso-level-number-format:bullet;
mso-level-text:\F0B7;
mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.25in;
mso-ansi-font-size:10.0pt;
font-family:Symbol;}
@list l320
{mso-list-id:1654674875;
mso-list-type:simple;
mso-list-template-ids:-2141796192;}
@list l320:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;
mso-ansi-font-weight:bold;}
@list l321
{mso-list-id:1654990238;
mso-list-template-ids:-2004183134;}
@list l321:level1
{mso-level-tab-stop:.75in;
mso-level-number-position:left;
margin-left:.75in;
text-indent:-.5in;}
@list l322
{mso-list-id:1661496744;
mso-list-type:simple;
mso-list-template-ids:34004356;}
@list l322:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l323
{mso-list-id:1664091494;
mso-list-type:simple;
mso-list-template-ids:-1889623146;}
@list l323:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l324
{mso-list-id:1665282169;
mso-list-template-ids:330344114;}
@list l324:level1
{mso-level-tab-stop:.75in;
mso-level-number-position:left;
margin-left:.75in;
text-indent:-.5in;}
@list l325
{mso-list-id:1680810861;
mso-list-type:simple;
mso-list-template-ids:-1271920838;}
@list l325:level1
{mso-level-start-at:7;
mso-level-text:%1;
mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;}
@list l326
{mso-list-id:1684550051;
mso-list-type:simple;
mso-list-template-ids:-383713286;}
@list l326:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l327
{mso-list-id:1696811589;
mso-list-type:simple;
mso-list-template-ids:67698689;}
@list l327:level1
{mso-level-number-format:bullet;
mso-level-text:\F0B7;
mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;
font-family:Symbol;}
@list l328
{mso-list-id:1699306857;
mso-list-type:hybrid;
mso-list-template-ids:1106643230;}
@list l328:level1
{mso-level-tab-stop:.75in;
mso-level-number-position:left;
margin-left:.75in;
text-indent:-.5in;}
@list l329
{mso-list-id:1702323554;
mso-list-template-ids:-652812694;}
@list l329:level1
{mso-level-tab-stop:.75in;
mso-level-number-position:left;
margin-left:.75in;
text-indent:-.5in;}
@list l330
{mso-list-id:1703433714;
mso-list-type:simple;
mso-list-template-ids:-1;}
@list l330:level1
{mso-level-number-format:bullet;
mso-level-text:\F0B7;
mso-level-tab-stop:none;
mso-level-number-position:left;
mso-level-legacy:yes;
mso-level-legacy-indent:.25in;
mso-level-legacy-space:0in;
margin-left:1.75in;
text-indent:-.25in;
font-family:Symbol;}
@list l331
{mso-list-id:1715807827;
mso-list-type:simple;
mso-list-template-ids:-319936150;}
@list l331:level1
{mso-level-tab-stop:1.0in;
mso-level-number-position:left;
margin-left:1.0in;
text-indent:-.5in;}
@list l332
{mso-list-id:1724908288;
mso-list-type:hybrid;
mso-list-template-ids:2033761036 1075096902 67698713 67698715 67698703 67698713 67698715 67698703 67698713 67698715;}
@list l332:level1
{mso-level-tab-stop:.75in;
mso-level-number-position:left;
margin-left:.75in;
text-indent:-.5in;}
@list l333
{mso-list-id:1730879109;
mso-list-type:simple;
mso-list-template-ids:-988377902;}
@list l333:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l334
{mso-list-id:1736776296;
mso-list-type:simple;
mso-list-template-ids:1675234646;}
@list l334:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l335
{mso-list-id:1741322524;
mso-list-type:simple;
mso-list-template-ids:67698703;}
@list l335:level1
{mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;}
@list l336
{mso-list-id:1742948774;
mso-list-type:simple;
mso-list-template-ids:67698689;}
@list l336:level1
{mso-level-number-format:bullet;
mso-level-text:\F0B7;
mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;
font-family:Symbol;}
@list l337
{mso-list-id:1743259181;
mso-list-type:simple;
mso-list-template-ids:67698689;}
@list l337:level1
{mso-level-number-format:bullet;
mso-level-text:\F0B7;
mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;
font-family:Symbol;}
@list l338
{mso-list-id:1744179327;
mso-list-type:simple;
mso-list-template-ids:-710933498;}
@list l338:level1
{mso-level-start-at:7;
mso-level-text:%1;
mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l339
{mso-list-id:1744840340;
mso-list-type:simple;
mso-list-template-ids:1760340070;}
@list l339:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l340
{mso-list-id:1745957416;
mso-list-type:simple;
mso-list-template-ids:67698689;}
@list l340:level1
{mso-level-number-format:bullet;
mso-level-text:\F0B7;
mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;
font-family:Symbol;}
@list l341
{mso-list-id:1749575766;
mso-list-type:simple;
mso-list-template-ids:67698703;}
@list l341:level1
{mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;}
@list l342
{mso-list-id:1752265795;
mso-list-template-ids:-652812694;}
@list l342:level1
{mso-level-tab-stop:.75in;
mso-level-number-position:left;
margin-left:.75in;
text-indent:-.5in;}
@list l343
{mso-list-id:1757248239;
mso-list-type:simple;
mso-list-template-ids:67698703;}
@list l343:level1
{mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;}
@list l344
{mso-list-id:1760634043;
mso-list-type:simple;
mso-list-template-ids:-1814925126;}
@list l344:level1
{mso-level-start-at:2;
mso-level-number-format:roman-lower;
mso-level-text:"\(%1\)";
mso-level-tab-stop:1.0in;
mso-level-number-position:left;
margin-left:1.0in;
text-indent:-.5in;}
@list l345
{mso-list-id:1763526252;
mso-list-type:simple;
mso-list-template-ids:67698699;}
@list l345:level1
{mso-level-number-format:bullet;
mso-level-text:\F0D8;
mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;
font-family:Wingdings;}
@list l346
{mso-list-id:1769351297;
mso-list-type:simple;
mso-list-template-ids:347772384;}
@list l346:level1
{mso-level-start-at:4;
mso-level-text:%1;
mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l347
{mso-list-id:1775397895;
mso-list-type:simple;
mso-list-template-ids:67698689;}
@list l347:level1
{mso-level-number-format:bullet;
mso-level-text:\F0B7;
mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;
font-family:Symbol;}
@list l348
{mso-list-id:1780176228;
mso-list-type:simple;
mso-list-template-ids:1259488058;}
@list l348:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l349
{mso-list-id:1784567270;
mso-list-type:simple;
mso-list-template-ids:67698703;}
@list l349:level1
{mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;}
@list l350
{mso-list-id:1786272282;
mso-list-type:hybrid;
mso-list-template-ids:-544187522 1075096902 67698713 67698715 67698703 67698713 67698715 67698703 67698713 67698715;}
@list l350:level1
{mso-level-tab-stop:.75in;
mso-level-number-position:left;
margin-left:.75in;
text-indent:-.5in;}
@list l351
{mso-list-id:1802578801;
mso-list-type:simple;
mso-list-template-ids:-2098060564;}
@list l351:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l352
{mso-list-id:1803229393;
mso-list-type:hybrid;
mso-list-template-ids:1763578978 1075096902 67698713 67698715 67698703 67698713 67698715 67698703 67698713 67698715;}
@list l352:level1
{mso-level-tab-stop:.75in;
mso-level-number-position:left;
margin-left:.75in;
text-indent:-.5in;}
@list l353
{mso-list-id:1804157683;
mso-list-type:simple;
mso-list-template-ids:901647176;}
@list l353:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l354
{mso-list-id:1807551140;
mso-list-type:simple;
mso-list-template-ids:-1437822026;}
@list l354:level1
{mso-level-start-at:8;
mso-level-tab-stop:39.0pt;
mso-level-number-position:left;
margin-left:39.0pt;
text-indent:-39.0pt;}
@list l355
{mso-list-id:1808740632;
mso-list-type:hybrid;
mso-list-template-ids:1220949254 1075096902 67698713 67698715 67698703 67698713 67698715 67698703 67698713 67698715;}
@list l355:level1
{mso-level-tab-stop:.75in;
mso-level-number-position:left;
margin-left:.75in;
text-indent:-.5in;}
@list l356
{mso-list-id:1825122962;
mso-list-type:simple;
mso-list-template-ids:67698689;}
@list l356:level1
{mso-level-number-format:bullet;
mso-level-text:\F0B7;
mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;
font-family:Symbol;}
@list l357
{mso-list-id:1826166046;
mso-list-type:simple;
mso-list-template-ids:-1003567320;}
@list l357:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l358
{mso-list-id:1829907690;
mso-list-type:simple;
mso-list-template-ids:67698703;}
@list l358:level1
{mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;}
@list l359
{mso-list-id:1830977161;
mso-list-type:simple;
mso-list-template-ids:-988377902;}
@list l359:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l360
{mso-list-id:1836146159;
mso-list-type:simple;
mso-list-template-ids:67698689;}
@list l360:level1
{mso-level-number-format:bullet;
mso-level-text:\F0B7;
mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;
font-family:Symbol;}
@list l361
{mso-list-id:1841122627;
mso-list-type:simple;
mso-list-template-ids:67698689;}
@list l361:level1
{mso-level-number-format:bullet;
mso-level-text:\F0B7;
mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;
font-family:Symbol;}
@list l362
{mso-list-id:1843200720;
mso-list-type:simple;
mso-list-template-ids:990390424;}
@list l362:level1
{mso-level-start-at:10;
mso-level-text:%1;
mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;}
@list l363
{mso-list-id:1843664903;
mso-list-type:simple;
mso-list-template-ids:-710933498;}
@list l363:level1
{mso-level-start-at:7;
mso-level-text:%1;
mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l364
{mso-list-id:1844272447;
mso-list-type:simple;
mso-list-template-ids:458537720;}
@list l364:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;
mso-ansi-font-weight:bold;}
@list l365
{mso-list-id:1855459450;
mso-list-type:simple;
mso-list-template-ids:67698699;}
@list l365:level1
{mso-level-number-format:bullet;
mso-level-text:\F0D8;
mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;
font-family:Wingdings;}
@list l366
{mso-list-id:1858739599;
mso-list-type:simple;
mso-list-template-ids:67698689;}
@list l366:level1
{mso-level-number-format:bullet;
mso-level-text:\F0B7;
mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;
font-family:Symbol;}
@list l367
{mso-list-id:1860964975;
mso-list-type:simple;
mso-list-template-ids:-2098060564;}
@list l367:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l368
{mso-list-id:1864971745;
mso-list-type:hybrid;
mso-list-template-ids:-1000171668;}
@list l368:level1
{mso-level-tab-stop:.75in;
mso-level-number-position:left;
margin-left:.75in;
text-indent:-.5in;}
@list l369
{mso-list-id:1872300047;
mso-list-type:simple;
mso-list-template-ids:67698689;}
@list l369:level1
{mso-level-number-format:bullet;
mso-level-text:\F0B7;
mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;
font-family:Symbol;}
@list l370
{mso-list-id:1875463398;
mso-list-type:simple;
mso-list-template-ids:67698703;}
@list l370:level1
{mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;}
@list l371
{mso-list-id:1878544858;
mso-list-type:simple;
mso-list-template-ids:67698703;}
@list l371:level1
{mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;}
@list l372
{mso-list-id:1886402229;
mso-list-type:simple;
mso-list-template-ids:-438120098;}
@list l372:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;
mso-ansi-font-weight:bold;}
@list l373
{mso-list-id:1901359022;
mso-list-template-ids:-2024758574;}
@list l373:level1
{mso-level-tab-stop:.75in;
mso-level-number-position:left;
margin-left:.75in;
text-indent:-.5in;}
@list l374
{mso-list-id:1904871678;
mso-list-template-ids:-764609236;}
@list l374:level1
{mso-level-start-at:8;
mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.25in;}
@list l374:level2
{mso-level-number-format:alpha-lower;
mso-level-tab-stop:1.0in;
mso-level-number-position:left;
text-indent:-.25in;}
@list l375
{mso-list-id:1909538490;
mso-list-type:hybrid;
mso-list-template-ids:686729236 1075096902 67698713 67698715 67698703 67698713 67698715 67698703 67698713 67698715;}
@list l375:level1
{mso-level-tab-stop:.75in;
mso-level-number-position:left;
margin-left:.75in;
text-indent:-.5in;}
@list l376
{mso-list-id:1914509020;
mso-list-type:simple;
mso-list-template-ids:1066541746;}
@list l376:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l377
{mso-list-id:1920164658;
mso-list-type:simple;
mso-list-template-ids:-2058609270;}
@list l377:level1
{mso-level-start-at:9;
mso-level-text:%1;
mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;}
@list l378
{mso-list-id:1920627368;
mso-list-type:simple;
mso-list-template-ids:67698689;}
@list l378:level1
{mso-level-number-format:bullet;
mso-level-text:\F0B7;
mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;
font-family:Symbol;}
@list l379
{mso-list-id:1927418889;
mso-list-type:simple;
mso-list-template-ids:67698703;}
@list l379:level1
{mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;}
@list l380
{mso-list-id:1934051206;
mso-list-type:simple;
mso-list-template-ids:67698689;}
@list l380:level1
{mso-level-number-format:bullet;
mso-level-text:\F0B7;
mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;
font-family:Symbol;}
@list l381
{mso-list-id:1937788222;
mso-list-type:simple;
mso-list-template-ids:-988377902;}
@list l381:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l382
{mso-list-id:1939408735;
mso-list-type:simple;
mso-list-template-ids:67698703;}
@list l382:level1
{mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;}
@list l383
{mso-list-id:1946188606;
mso-list-type:simple;
mso-list-template-ids:-1147497058;}
@list l383:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l384
{mso-list-id:1949046532;
mso-list-template-ids:-671316730;}
@list l384:level1
{mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;}
@list l384:level2
{mso-level-legal-format:yes;
mso-level-text:"%1\.%2";
mso-level-tab-stop:51.75pt;
mso-level-number-position:left;
margin-left:51.75pt;
text-indent:-36.75pt;}
@list l384:level3
{mso-level-legal-format:yes;
mso-level-text:"%1\.%2\.%3";
mso-level-tab-stop:66.75pt;
mso-level-number-position:left;
margin-left:66.75pt;
text-indent:-36.75pt;}
@list l384:level4
{mso-level-legal-format:yes;
mso-level-text:"%1\.%2\.%3\.%4";
mso-level-tab-stop:99.0pt;
mso-level-number-position:left;
margin-left:99.0pt;
text-indent:-.75in;}
@list l384:level5
{mso-level-legal-format:yes;
mso-level-text:"%1\.%2\.%3\.%4\.%5";
mso-level-tab-stop:132.0pt;
mso-level-number-position:left;
margin-left:132.0pt;
text-indent:-1.0in;}
@list l384:level6
{mso-level-legal-format:yes;
mso-level-text:"%1\.%2\.%3\.%4\.%5\.%6";
mso-level-tab-stop:147.0pt;
mso-level-number-position:left;
margin-left:147.0pt;
text-indent:-1.0in;}
@list l384:level7
{mso-level-legal-format:yes;
mso-level-text:"%1\.%2\.%3\.%4\.%5\.%6\.%7";
mso-level-tab-stop:2.5in;
mso-level-number-position:left;
margin-left:2.5in;
text-indent:-1.25in;}
@list l384:level8
{mso-level-legal-format:yes;
mso-level-text:"%1\.%2\.%3\.%4\.%5\.%6\.%7\.%8";
mso-level-tab-stop:213.0pt;
mso-level-number-position:left;
margin-left:213.0pt;
text-indent:-1.5in;}
@list l384:level9
{mso-level-legal-format:yes;
mso-level-text:"%1\.%2\.%3\.%4\.%5\.%6\.%7\.%8\.%9";
mso-level-tab-stop:228.0pt;
mso-level-number-position:left;
margin-left:228.0pt;
text-indent:-1.5in;}
@list l385
{mso-list-id:1951862522;
mso-list-type:simple;
mso-list-template-ids:-1684354048;}
@list l385:level1
{mso-level-number-format:alpha-lower;
mso-level-text:"\(%1\)";
mso-level-tab-stop:.75in;
mso-level-number-position:left;
margin-left:.75in;
text-indent:-.25in;}
@list l386
{mso-list-id:1956715411;
mso-list-type:simple;
mso-list-template-ids:81568798;}
@list l386:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l387
{mso-list-id:1959944020;
mso-list-type:simple;
mso-list-template-ids:67698689;}
@list l387:level1
{mso-level-number-format:bullet;
mso-level-text:\F0B7;
mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;
font-family:Symbol;}
@list l388
{mso-list-id:1960142366;
mso-list-type:hybrid;
mso-list-template-ids:-1008820826;}
@list l388:level1
{mso-level-tab-stop:.75in;
mso-level-number-position:left;
margin-left:.75in;
text-indent:-.5in;}
@list l389
{mso-list-id:1964072409;
mso-list-type:hybrid;
mso-list-template-ids:-1474123906 1075096902 67698713 67698715 67698703 67698713 67698715 67698703 67698713 67698715;}
@list l389:level1
{mso-level-tab-stop:.75in;
mso-level-number-position:left;
margin-left:.75in;
text-indent:-.5in;}
@list l390
{mso-list-id:1965959276;
mso-list-type:simple;
mso-list-template-ids:-663612576;}
@list l390:level1
{mso-level-start-at:119;
mso-level-tab-stop:21.0pt;
mso-level-number-position:left;
margin-left:21.0pt;
text-indent:-21.0pt;}
@list l391
{mso-list-id:1969512715;
mso-list-type:simple;
mso-list-template-ids:1467091562;}
@list l391:level1
{mso-level-number-format:alpha-lower;
mso-level-text:"\(%1\)";
mso-level-tab-stop:1.0in;
mso-level-number-position:left;
margin-left:1.0in;
text-indent:-.5in;}
@list l392
{mso-list-id:1971478504;
mso-list-type:simple;
mso-list-template-ids:67698689;}
@list l392:level1
{mso-level-number-format:bullet;
mso-level-text:\F0B7;
mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;
font-family:Symbol;}
@list l393
{mso-list-id:1982954631;
mso-list-type:simple;
mso-list-template-ids:-988377902;}
@list l393:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l394
{mso-list-id:1985236501;
mso-list-type:simple;
mso-list-template-ids:-988377902;}
@list l394:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l395
{mso-list-id:1987346272;
mso-list-type:simple;
mso-list-template-ids:-1297197190;}
@list l395:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l396
{mso-list-id:1991322803;
mso-list-type:simple;
mso-list-template-ids:-988377902;}
@list l396:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l397
{mso-list-id:1993096506;
mso-list-type:simple;
mso-list-template-ids:624745472;}
@list l397:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l398
{mso-list-id:1997371293;
mso-list-type:simple;
mso-list-template-ids:523773134;}
@list l398:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l399
{mso-list-id:2001342891;
mso-list-type:simple;
mso-list-template-ids:67698689;}
@list l399:level1
{mso-level-number-format:bullet;
mso-level-text:\F0B7;
mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;
font-family:Symbol;}
@list l400
{mso-list-id:2008314957;
mso-list-type:simple;
mso-list-template-ids:-26316910;}
@list l400:level1
{mso-level-start-at:5;
mso-level-text:"";
mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;}
@list l401
{mso-list-id:2011134345;
mso-list-type:simple;
mso-list-template-ids:-289273672;}
@list l401:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l402
{mso-list-id:2028367403;
mso-list-type:hybrid;
mso-list-template-ids:1792948066 67698703 67698713 67698715 67698703 67698713 67698715 67698703 67698713 67698715;}
@list l402:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.25in;}
@list l403
{mso-list-id:2036274003;
mso-list-type:simple;
mso-list-template-ids:67698689;}
@list l403:level1
{mso-level-number-format:bullet;
mso-level-text:\F0B7;
mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;
font-family:Symbol;}
@list l404
{mso-list-id:2037926632;
mso-list-template-ids:-1163229930;}
@list l404:level1
{mso-level-tab-stop:.75in;
mso-level-number-position:left;
margin-left:.75in;
text-indent:-.5in;}
@list l405
{mso-list-id:2042893811;
mso-list-type:simple;
mso-list-template-ids:-1696197602;}
@list l405:level1
{mso-level-number-format:alpha-lower;
mso-level-text:"\(%1\)";
mso-level-tab-stop:1.0in;
mso-level-number-position:left;
margin-left:1.0in;
text-indent:-.5in;}
@list l406
{mso-list-id:2043170469;
mso-list-template-ids:416302836;}
@list l406:level1
{mso-level-number-format:roman-upper;
mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;
mso-ansi-font-weight:bold;}
@list l406:level2
{mso-level-legal-format:yes;
mso-level-text:"%1\.%2";
mso-level-tab-stop:51.75pt;
mso-level-number-position:left;
margin-left:51.75pt;
text-indent:-36.75pt;}
@list l406:level3
{mso-level-legal-format:yes;
mso-level-text:"%1\.%2\.%3";
mso-level-tab-stop:66.75pt;
mso-level-number-position:left;
margin-left:66.75pt;
text-indent:-36.75pt;}
@list l406:level4
{mso-level-legal-format:yes;
mso-level-text:"%1\.%2\.%3\.%4";
mso-level-tab-stop:99.0pt;
mso-level-number-position:left;
margin-left:99.0pt;
text-indent:-.75in;}
@list l406:level5
{mso-level-legal-format:yes;
mso-level-text:"%1\.%2\.%3\.%4\.%5";
mso-level-tab-stop:132.0pt;
mso-level-number-position:left;
margin-left:132.0pt;
text-indent:-1.0in;}
@list l406:level6
{mso-level-legal-format:yes;
mso-level-text:"%1\.%2\.%3\.%4\.%5\.%6";
mso-level-tab-stop:147.0pt;
mso-level-number-position:left;
margin-left:147.0pt;
text-indent:-1.0in;}
@list l406:level7
{mso-level-legal-format:yes;
mso-level-text:"%1\.%2\.%3\.%4\.%5\.%6\.%7";
mso-level-tab-stop:2.5in;
mso-level-number-position:left;
margin-left:2.5in;
text-indent:-1.25in;}
@list l406:level8
{mso-level-legal-format:yes;
mso-level-text:"%1\.%2\.%3\.%4\.%5\.%6\.%7\.%8";
mso-level-tab-stop:213.0pt;
mso-level-number-position:left;
margin-left:213.0pt;
text-indent:-1.5in;}
@list l406:level9
{mso-level-legal-format:yes;
mso-level-text:"%1\.%2\.%3\.%4\.%5\.%6\.%7\.%8\.%9";
mso-level-tab-stop:228.0pt;
mso-level-number-position:left;
margin-left:228.0pt;
text-indent:-1.5in;}
@list l407
{mso-list-id:2043363741;
mso-list-type:simple;
mso-list-template-ids:-609956022;}
@list l407:level1
{mso-level-start-at:118;
mso-level-text:%1;
mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l408
{mso-list-id:2056153563;
mso-list-type:simple;
mso-list-template-ids:-1929474642;}
@list l408:level1
{mso-level-start-at:4;
mso-level-number-format:bullet;
mso-level-text:-;
mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l409
{mso-list-id:2057390033;
mso-list-template-ids:330344114;}
@list l409:level1
{mso-level-tab-stop:.75in;
mso-level-number-position:left;
margin-left:.75in;
text-indent:-.5in;}
@list l410
{mso-list-id:2061779757;
mso-list-type:simple;
mso-list-template-ids:67698689;}
@list l410:level1
{mso-level-number-format:bullet;
mso-level-text:\F0B7;
mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;
font-family:Symbol;}
@list l411
{mso-list-id:2073766262;
mso-list-type:simple;
mso-list-template-ids:67698703;}
@list l411:level1
{mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;}
@list l412
{mso-list-id:2087073178;
mso-list-type:simple;
mso-list-template-ids:67698703;}
@list l412:level1
{mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;}
@list l413
{mso-list-id:2091538554;
mso-list-template-ids:-652812694;}
@list l413:level1
{mso-level-tab-stop:.75in;
mso-level-number-position:left;
margin-left:.75in;
text-indent:-.5in;}
@list l414
{mso-list-id:2093234781;
mso-list-type:simple;
mso-list-template-ids:-1568780276;}
@list l414:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l415
{mso-list-id:2095127258;
mso-list-type:simple;
mso-list-template-ids:67698703;}
@list l415:level1
{mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;}
@list l416
{mso-list-id:2105224327;
mso-list-type:simple;
mso-list-template-ids:67698689;}
@list l416:level1
{mso-level-number-format:bullet;
mso-level-text:\F0B7;
mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;
font-family:Symbol;}
@list l417
{mso-list-id:2110537337;
mso-list-template-ids:-652812694;}
@list l417:level1
{mso-level-tab-stop:.75in;
mso-level-number-position:left;
margin-left:.75in;
text-indent:-.5in;}
@list l418
{mso-list-id:2114203490;
mso-list-type:simple;
mso-list-template-ids:-988377902;}
@list l418:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l419
{mso-list-id:2121143942;
mso-list-type:simple;
mso-list-template-ids:-1;}
@list l419:level1
{mso-level-number-format:bullet;
mso-level-text:\F0B7;
mso-level-tab-stop:none;
mso-level-number-position:left;
mso-level-legacy:yes;
mso-level-legacy-indent:.25in;
mso-level-legacy-space:0in;
margin-left:1.75in;
text-indent:-.25in;
font-family:Symbol;}
@list l420
{mso-list-id:2122262329;
mso-list-template-ids:-652812694;}
@list l420:level1
{mso-level-tab-stop:.75in;
mso-level-number-position:left;
margin-left:.75in;
text-indent:-.5in;}
@list l421
{mso-list-id:2124113205;
mso-list-type:simple;
mso-list-template-ids:67698703;}
@list l421:level1
{mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;}
@list l422
{mso-list-id:2126267715;
mso-list-type:simple;
mso-list-template-ids:-1087596128;}
@list l422:level1
{mso-level-start-at:36;
mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l423
{mso-list-id:2128696928;
mso-list-type:simple;
mso-list-template-ids:67698703;}
@list l423:level1
{mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;}
@list l424
{mso-list-id:2130390066;
mso-list-type:simple;
mso-list-template-ids:67698689;}
@list l424:level1
{mso-level-number-format:bullet;
mso-level-text:\F0B7;
mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;
font-family:Symbol;}
@list l425
{mso-list-id:2133480798;
mso-list-type:simple;
mso-list-template-ids:-988377902;}
@list l425:level1
{mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l426
{mso-list-id:2136940980;
mso-list-template-ids:-671316730;}
@list l426:level1
{mso-level-tab-stop:.25in;
mso-level-number-position:left;
margin-left:.25in;
text-indent:-.25in;}
@list l426:level2
{mso-level-legal-format:yes;
mso-level-text:"%1\.%2";
mso-level-tab-stop:51.75pt;
mso-level-number-position:left;
margin-left:51.75pt;
text-indent:-36.75pt;}
@list l426:level3
{mso-level-legal-format:yes;
mso-level-text:"%1\.%2\.%3";
mso-level-tab-stop:66.75pt;
mso-level-number-position:left;
margin-left:66.75pt;
text-indent:-36.75pt;}
@list l426:level4
{mso-level-legal-format:yes;
mso-level-text:"%1\.%2\.%3\.%4";
mso-level-tab-stop:99.0pt;
mso-level-number-position:left;
margin-left:99.0pt;
text-indent:-.75in;}
@list l426:level5
{mso-level-legal-format:yes;
mso-level-text:"%1\.%2\.%3\.%4\.%5";
mso-level-tab-stop:132.0pt;
mso-level-number-position:left;
margin-left:132.0pt;
text-indent:-1.0in;}
@list l426:level6
{mso-level-legal-format:yes;
mso-level-text:"%1\.%2\.%3\.%4\.%5\.%6";
mso-level-tab-stop:147.0pt;
mso-level-number-position:left;
margin-left:147.0pt;
text-indent:-1.0in;}
@list l426:level7
{mso-level-legal-format:yes;
mso-level-text:"%1\.%2\.%3\.%4\.%5\.%6\.%7";
mso-level-tab-stop:2.5in;
mso-level-number-position:left;
margin-left:2.5in;
text-indent:-1.25in;}
@list l426:level8
{mso-level-legal-format:yes;
mso-level-text:"%1\.%2\.%3\.%4\.%5\.%6\.%7\.%8";
mso-level-tab-stop:213.0pt;
mso-level-number-position:left;
margin-left:213.0pt;
text-indent:-1.5in;}
@list l426:level9
{mso-level-legal-format:yes;
mso-level-text:"%1\.%2\.%3\.%4\.%5\.%6\.%7\.%8\.%9";
mso-level-tab-stop:228.0pt;
mso-level-number-position:left;
margin-left:228.0pt;
text-indent:-1.5in;}
@list l427
{mso-list-id:2140494840;
mso-list-type:simple;
mso-list-template-ids:-1361413676;}
@list l427:level1
{mso-level-start-at:17;
mso-level-tab-stop:.75in;
mso-level-number-position:left;
margin-left:.75in;
text-indent:-.25in;}
@list l428
{mso-list-id:2144037790;
mso-list-type:simple;
mso-list-template-ids:-1630918638;}
@list l428:level1
{mso-level-number-format:alpha-lower;
mso-level-text:"%1\)";
mso-level-tab-stop:.5in;
mso-level-number-position:left;
text-indent:-.5in;}
@list l316:level1 lfo324
{mso-level-start-at:13;}
ol
{margin-bottom:0in;}
ul
{margin-bottom:0in;}
-->
|
JUNE 2004
SCHEME
OF EXAMINATION
&
SYLLABUS
FOR B.Tech.
in
Biotechnology
YEAR
2004 ONWARDS
UNIVERSITY SCHOOL OF
BIOTECHNOLOGY
GGS
INDRAPRASTHA UNIVERSITY
KASHMERE
GATE
DELHI
- 110 006
|
SCHEME OF EXAMINATION FOR MASTER OF BIOTECHNOLOGY
L T P
S Credits Hours
15 5 12
0 26 32
FIRST SEMESTER EXAMINATION
Code No. L
T/P Credits
THEORY
PAPERS
BA-121 Foundation course in
Physico-Inorganic Chemistry I
BA-123 Foundation course in Physics-I
BA-113 Life Sciences I
BA-109 Mathematics I
BT-115 Concepts in Biotechnology
IT-
105 Introduction to Computers
Practicals :
BA-155 Chemistry - I Lab
BA-153 Physics - I Lab
BA-159 Life Sciences I Lab
BT-161 Biotechnology Lab
IT
-155 Computer Lab
IT
-157 Engineering Graphics - I
Lab
|
2
2
3
3
2
3
0
0
0
0
0
0
|
1
1
1
1
1
0
2
2
2
2
2
2
|
3
3
4
4
3
3
1
1
1
1
1
1
|
|
TOTAL
|
15
|
5/12
|
26
|
SECOND SEMESTER
EXAMINATION
L T P S Credits Hours
16 6
10 0 27
32
Code No. L T/P
Credits
THEORY
PAPERS
BA-128 Foundation Course in Physics-II
BA-108 Mathematics-II
BA-132 Foundation Course in Organic
Chemistry-II
BA-122 Life Sciences II
BT-124 Laboratory Techniques in
Biotechnology
IT
-120 Electrical Science
Practicals :
BA-156 Physics - II Lab
BA-166 Chemistry II Lab
BA-168 Life Sciences - II LAb
BT-164 Laboratory Techniques in
Biotechnology -Lab
IT
-166 Electrical Science-Lab
|
2
3
2
3
3
3
0
0
0
0
0
|
1
1
1
1
1
1
2
2
2
2
2
|
3
4
3
4
4
4
1
1
1
1
1
|
|
TOTAL
|
16
|
6/10
|
27
|
THIRD SEMESTER
EXAMINATION
L T P S Credits Hours
15 6 10 0 29 34
Code No. L T/P Credits
THEORY
PAPERS
BT-201 Microbiology
BA-203 Bioenergetics I
BT-205 Cell Biology
BT-209 Genetics
BT-211 Biostatistics
CT-211 Chemical Engineering-I
Practicals:
BA-253 Bioenergetics I Lab
BT-251 Genetics-Lab
BT-255 Cell Biology-Lab
BT-257 Microbiology lab
|
2
3
3
2
2
3
0
0
0
0
|
1
1
1
1
1
1
3
4
3
3
|
3
4
4
3
3
4
2
2
2
2
|
|
TOTAL
|
15
|
6/13
|
29
|
FOURTH SEMESTER
EXAMINATION
L T P S Credits Hours
14 5 13 0 26 32
Code No. L T/P Credits
THEORY
PAPERS
BT-202
Immunology
BT-204 Molecular Biology
BT-206 Enzyme Technology
BA-208 Bioenergetics II
CT-212 Chemical Engineering-II
Practicals:
BT-254 Molecular Biology -Lab
BT-256 Enzyme Technology-Lab
BA-258 Bioenergetics - II Lab
BT-258 Immunology Lab
|
2
3
3
3
3
0
0
0
0
|
1
1
1
1
1
4
4
3
2
|
3
4
4
4
4
2
2
2
1
|
|
TOTAL
|
14
|
5/13
|
26
|
FIFTH SEMESTER EXAMINATION
L T P S Credits Hours
12 5
12 0 27 32
Code No. L T/P Credits
THEORY
PAPERS
BT-301 Plant Tissue Culture
BT-303 Microbial Processing Engineering
BT-305 Animal Biotechnology
BT-307 Recombinant DNA Technology
BT-309 Developmental Biology
Practicals:
BT-351 Plant tissue Culture -Lab
CT-361 Chemical Engineering -Lab
BT-355 Animal tissue Culture -Lab
BT-357 Rec. DNA Tech. & Appl._Lab
BT-359 Developmental Biology Lab
|
2
3
3
2
2
0
0
0
0
0
|
1
1
1
1
1
3
3
3
3
3
|
3
4
4
3
3
2
2
2
2
2
|
|
TOTAL
|
12
|
5/15
|
27
|
SIXTH SEMESTER
EXAMINATION
L T P S Credits Hours
14 6 12 0 28 32
Code No. L T/P Credits
THEORY
PAPERS
BT-302 Bioinformatics
BT-304 Food Biotechnology
BT-306 Plant Biotechnology
BT-308 Down Stream Processing
BT-310 Biosensor
BT-312 Biochemical Engineering & Biotechnology
(Elective for CT
Students)
Practicals:
BT-352 Bioinformatics -Lab
BT-354 Food Biotechnology -Lab
BT-356 Plant Biotechnology -Lab
BT-358 Downstream / Microbial
Processing-Lab
|
3
2
2
3
2
2
0
0
0
0
|
1
1
1
1
1
1
3
3
3
3
|
4
3
3
4
3
3
2
2
2
2
|
|
TOTAL
|
14
|
6/12
|
28
|
SEVENTH SEMESTER
EXAMINATION
L T P S Credits Hours
13 5 12
0 25 30
Code No. L T/P
Credits
|
THEORY PAPERS
BT-401 Stem Cells in Health Care
BT-403 Environmental Biotechnology
BT-405 Protein Biotechnology
BT-407 Commercialization, Marketing and
Management of
Biotechnological Products
HS-409 Writing Skills for Technical
Purposes
Practicals:
BT-451 Protein Biotechnology-Lab
BT-453 Environmental Biotechnology -Lab
BT-455 Field Trips
|
3
2
2
3
3
0
0
0
|
1
1
1
1
1
3
2
7
|
4
3
3
4
4
2
1
4
|
|
TOTAL
|
13
|
5/12
|
25
|
EIGHTH SEMESTER
EXAMINATION
L T P S Credits Hours
9 3 18
0 30 30
Code No. L T/P
Credits
THEORY
PAPERS
BT-402 Industrial Biotechnology
BT-404 Intellectual Property Rights in
Biotechnology
BT-406 Molecular & Cellular
Diagnostics
BT-408 Biosafety & Bioethics
Project workl/Viva-voce :
BT-450 Project Work
|
3
2
2
2
0
|
1
1
1
0
18
|
4
3
3
2
18
|
|
TOTAL
|
9
|
3/18
|
30
|
BA-121 FOUNDATION
COURSE IN PHYSICO INORGANIC CHEMISTRY - I
1.
Chemical Bonding: Ionic
bond ; energy changes, lattice energy Born Haber Cycle, Covalent bond-energy
changes, Potential energy curve for H2
Molecule, characteristics of covalent compound, co-ordinate bond -
Werner's Theory, effective atomic numbers, isomerism in coordinate
compounds. Hydrogen bonding, Vander
Waal's forces, hybridisation and resonance, Valance Shell Electron Repulsion
theory (VSEPR). Discussion of
structures of H2O, NH3, SiF4. Molecular orbital theory, Linear combination
of atomic orbitals (LCAO) method.
Structure of simple homo nuclear diatomic molecule like H2, N2,
O2, F2.
2.
Thermochemistry:
Hess's Law, heat of a reaction, effect of temperature on heat of reaction, at
constant pressure (Kirchoff's Equation) heat of dilution, heat of hydration,
heat of neutralization and heat of combustion, Flame temperature.
3.
Reaction Kinetics:
Significance of rate law and rate equations, order and molecularity,
Determinations of order of simple reactions-experimental method, Equilibrium
constant and reaction rates-Lindemann, collision and activated complex
theories, complex reactions of Ist order characterstics of consecutive,
reversible and parallel reactions-Steady state and non-steady state approach.
4.
Catalysis:
Criteria for Catalysis - Homogeneous Catalysis, acid-base, Enzymatic catalysis,
Catalysis by metal salts, Heterogeneous catalysis - concepts of promoters,
inhibitors and poisoning, Physiosorption, Chemisorption, Surface area,
Industrially important process.
Theories of Catalysis.
5.
Polymers: Basic
concepts & Terminology, such as monomers, Polymers, Functionality,
Thermoplastics, Thermosets Linear, Branched, cross linked polymers etc. different definitions of molecular weight
viz., Mw, Mn, Mv and then determinations, Industrial applications of polymers,
Addition, condensation and Ionic polymerization's, solutions of polymers, good
solvents, & bad solvent, solubility parameter, solutions viscosity and
determination of intrinsic viscosity.
6.
Colloids: Collidal
state, classification of colloidal solution, true solution, colloidal solution
and suspensions, preparation of sol, Purification of colloidal solutions,
General properites and optical properites, stability of colloids, coagulation
of lyphobic sols, electrical properties of sols, kinetic properties of
colloids:- Brownion movement, size of colloidal particle, emulsions, gels,
colloidal electrolytes and applications of colloids.
Text / Reference Books
1.
Inorganic Chemistry
by J.D. Lee.
2.
Physical Chemistry by
Lewis.
BA-123 FOUNDATION
COURSE IN PHYSICS - I
1.
Optics.
Interference: Coherence and
coherent sources, Interference by division of wavefront (Young's double slit
experiment, Fresnels' biprism), Interference by division of amplitude (Thin
films, Newton's rings,Michelson's Interferrometer,Fabry Perot Interferrometer
2.
Diffraction: Fresnel
and Fraunhoffer types of diffraction, Fraunhofer diffraction: Single slit,
double slit, circular aperture and N-slit. Diffraction grating - wavelength
determination, resolving power and dispersive power. Resolving power of optical
instruments Rayleigh criterion. Fresnel Diffraction: zone plate, circular
aperture, opaque circular disc, narrow slit.
3.
Polarization: Types
of polarization, elliptically and circularly polarized light Brewster's law,
Malu's law, Nicol prism, double refraction, quarter-wave and half-wave plates,
optical activity, specific rotation, Laurent half-shade polarimeter.
4.
Lasers: Introduction,
Coherence, Einstein A and B coefficients, population inversion, Basic principle
and operation of a laser, Types of
lasers, He-Ne laser, Ruby laser, semi-conductor laser, holography - theory and
applications
5.
Introduction to Optical fibre: Types of optical fibres and their characteristics,
(Attenuation and Dispersion) step index and graded index fibres, principle of
fibre optic communication- total internal reflection, Numerical aperture, Fibre
optical communication network- its advantages. Fibre optic sensors
(qualitative)
6.
Nature of light and matter: Particle nature of radiation- The Photoelectric effect,
Compton effect. X-rays (continuous and
characteristic), x-ray diffraction- Bragg's law. The origin of quantum theory-
Planck's hypothesis, the wave nature of matter- wave-particle duality, matter
waves (de Broglie hypothesis). Basic postulates of quantum mechanics - the wave
function - its physical interpretation, the Schrodinger equation.
7.
The electromagnetic spectrum: Sources of light, emission and absorption spectra, Brief
introduction to spectroscopy (optical, magnetic resonance).
Text / Reference Books
1.
Modern Physics by A.
Beiser.
2.
Optics by A.K.
Ghetak.
3.
Modern Physics by
Hallday & Resvik.
4.
Introduction to
Physical Optics by Jenkin & White.
BA-113 LIFE
SCIENCES - I
1.
Origin of Life : History
of earth, theories of origin of life nature of the earliest organism.
2.
Varieties of life : Classification,
Five kingdoms, viruses (TMV, HIV, Bacteriophage), Prokaryote (Bacteria-cell
structure, nutrition, reproduction), Protista, Fungi, Plantae and Animalia.
3.
Chemicals of life : (Biomolecules)-
Carbohydrates lipids, amino acids, proteins, nucleic acids, identification of
biomolecules in tissues.
4.
Cell : The cell
concept, structure of prokaryotic and eukaryotic cells, plant cells and animal
cells, cell membrances, cell organelles and their function. Structure and use of compound microscope.
5.
Histology: Maritimes
(apical, intercalary, lateral) and their function; simple tissue (parenchyma,
collenchymas, sclerenchyma); Complex tissue (xylem and phloem); Tissue systems
(epidermal, ground, vascular); primary body and growth (root, stem, leaf);
Secondary growth. Animal Epithelial tissue, connective tissue, muscle tissue
and nervous tissue and their function in body.
6.
Nutrition: Autotrophic
(Photosynthesis) Pigment systems, Chloroplast, light absorption by chlorophyll
and transfer of energy, two pigment systems, photosynthetic unit,
phosphorylation and electron transport system, Calvin-Benson Cycle (C3),
Hatch Slack Pathway (C4), Crassulacan Acid Metabolism (CAM), factors
affecting photosynthesis; Mineral Nutrition in plants. Heterotrophic - Forms of
heterotrophic nutrition, elementary canal in humans, nervous and hormonal
control of digestive systems, fate of absorbed food materials; Nutrition in
humans, Reference values.
7.
Energy Utilization:
(Respiration) - Structure of mitochondria, cellular respiration, relationship
of carbohydrate metabolism to other compounds, Glycolysis, fermentation,
formation of acetyl co-A, Kreb cycle, Electron Transport System and Oxidative
Phosphorylation, ATP, factors affecting respiration.
8.
Transport: Plant
water relationships, properties of water, diffusion, osmosis, imbibition,
movement of water in flowering plants, uptake of water by roots, the ascent of
water in xylem, apoplast symplast theory, Transpiration-structure of leaf and
stomata in plants opening and closing mechanisim of stomata factors affecting
transpiration, significance of transpiration General characteristics of blood
vascular system, development of blood systems in animals, Composition of blood,
circulation in blood vessels, formation of tissue fluids, the heart, functions
of mammalian blood, the immune system.
Text
/ Reference Books
BA-109 MATHEMATICS
- I
1. Differentiation:
Successive Differentiation, Leibnitzs theorem (without proof). Lagranges
Theorem, Cauchy mean value theorem, Taylors theorem (without proof), Remainder
Term, Asymptotes, Curvature, Curve Tracing
2. Infinite Series : Convergence,
divergence, Comparison test, Ratio test, Cauchys nth root test
test, Leibnitzs test (without proof), Absolute and Conditional Convergence.
Taylor and Meclaurin series, Power series, Radius of convergence
3. Integral Calculus :
Reduction Formulae of trignometric functions,
Properties of definite Integral, Applications to length, area, volume, surface
of revolution, Definition of improper
integrals, Beta-Gamma functions.
4. Calculus of
Functions of Several Variables : Partial
derivatives, Chain rule, Differentiation of Implicit functions, Exact
differentials. Maxima, Minima and Saddle points. Method of Lagrange
multipliers. Differentiation under integral sign. Jacobians and transformations
of coordinates. Double and Triple integrals,
Simple applications to areas, volumes etc
5. Vector Calculus :
Scalar and vector fields. Curves, Arc length, Tangent, normal, Directional Derivative, Gradient of scalar field, divergence and curl
of a vector field. Line integrals (independent of path), Greens theorem,
Divergence theorem and Stokes theorem (without proofs), Surface Integrals.
Text / Reference Books
1.
G.B.
Thomas and R.L. Finney, Calculus and Analytic Geometry, 6th
edition, Addision Wesley/Narosa, 1985.
2.
Shanti Narayan,
Differential Calculus, S. Chand & Co.
3.
Shanti Narayan,
Integral Calculus, S.Chand & Co.
4.
Grewal B.S., Higher
Engineering Mathematics, Khanna Publication.
5.
E. Kreyszig,
Advanced Engineering Mathematics, 5th Edition, Wiley Eastern,
1985.
BT-115 CONCEPTS
IN BIOTECHNOLOGY
1. Introduction
to Biotechnology: Definitions,
Historical perspectives, Scope and importance, Commercial potential, An
interdisciplinary challenge, A Quantitative approach, Classical vs. Modern
concepts, Manufacturing quality control, Product safety, Good manufacturing
practices, Good laboratory practices, Marketing, Biotechnology in India and
Global trends
2. Fundamentals of Biochemical Engineering
- Concept of pH,
Buffer, Physical variables, dimensions and units, Measurement conventions,
Physical and chemical property data, Stoichiometry, Errors in data and
calculations, absolute and relative uncertainty, types of error, Statistical
analysis, Presentation of experimental data, Data analysis, Trends, Testing
mathematical models, goodness of Fit, Use of graph paper with Logarithmic
Coordinates, General procedure for plotting data, Process flow diagrams,
Material and energy balances, fluid flow and mixing, Heat transfer, Mass
transfer, Unit operations, Homogeneous reactions, Heterogeneous reactions,
Reactor engineering.
3. Protein Structure and
Engineering: Introduction to the world of Proteins, 3-D Shape of
Proteins, Structure Function relationship in Proteins, Purification of
Proteins, Characterization of Proteins, Protein based products, Designing
Proteins, Proteomics.
4. Recombinant DNA Technology: Introduction,
Tools of rDNA Technology, Making Recombinant DNA, DNA Library, Introduction of
Recombinant DNA into host cells, Identification of Recombinants, Polymerase
Chain Reaction (PCR), DNA Probes, Hybridization Techniques, DNA Sequencing,
Site-directed mutagenesis.
5. Genomics and Bioinformatics: Introduction,
Genome Sequencing Projects, Gene prediction and Counting, Genome similarity,
SNPs and comparative genomics, Functional Genomics, History of Bioinformatics,
Sequences and Nomenclature, Information Sources, Analysis using Bioinformatics
tools.
6. Microbial Culture and Applications: Introduction, Microbial Culture Techniques, Measurement and Kinetics of
Microbial Growth, Scale up of Microbial Process, Isolation of Microbial
Products, Strain Isolation and Improvement, Applications of Microbial Culture
Technology, Bioethics in Microbial Technology.
7. Plant Cell Culture and Application: Introduction, Cell and Tissue Culture Techniques, Applications of Cell
and Tissue Culture, Gene Transfer Methods in Plants, Transgenic Plants with
Beneficial Traits, Diagnostics in Agriculture and Molecular Breeding, Bioethics
in Plant Genetic Engineering.
8. Animal Cell Culture and Applications: Introduction, Animal Cell Culture Techniques, Characterisation of Cell
Lines, Scale-up of Animal Culture Process, Applications of Animal Cell Culture,
Stem Cell Technology, Bioethics in Animal Genetic Engineering.
9. Biotechnology
and Society - Public perception, Role of sciences, Engineering, Arts, Commerce, Patenting - Criterion for
patents, Discovery vs Invention, Product and process patent, Reading a patent,
National and International Patent Laws, Varietal protection, Patenting of
biological systems, Ethical issues in agriculture and health care
Text / Reference Books
1.
A textbook of
Biotechnology for Class XI and XII, CBSE.
2.
Biotechnology by
Smith, Cambridge Press.
IT 105 INTRODUCTION TO COMPUTERS
- Introduction: Overview of computer organization and
historical perspective computer applications in various fields of science
and management, Data representation: Number systems, character
representation codes, Binary, hex, octal codes and their inter
conversions. Binary arithmetic, floating point arithmetic, signed and
unsigned numbers. Data storage: Primary and Secondary storage.
Introduction to various computer devices such as keyboard, mouse,
printers, disk files, floppies etc. Concept of computing, contemporary,
Operating Systems such as DOS, Windows 95, UNIX etc. (only brief user
level description). Introduction to organization and architecture of
mainframe, mini and micro systems. Introduction to E-mail, ftp, login and
other network services, worldwide web, MS-Office.
- Introduction to Programming: Concept of algorithms, Flow
charts, Example of Algorithms such as how to add ten numbers roots of a
quadratic equation. Concept of
sequentially following up the steps of the algorithm, Notion of program,
programmability and programming languages. Structure of programs, object codes, compilers, Introduction
to the Editing tools such as vi or MS-VC editors, Concepts of the finite
storage, bits, bytes, kilo, mega and gigabytes. Concepts of character representation.
- Programming using C: The emphasis should be more on
programming techniques rather that the language itself. The C programming language is being
chosen mainly because of the availability of the compilers, books and
other reference materials, Example of some simple C program. Dissection of the program line by
line. Concepts of variables,
program statements and function calls from the library (print for
example), C data types, int, char, float etc, C expressions, arithmetic
operations, relational and logic operations, C assignment statements,
extension of assignment to the operations. C primitive input output using getchar and putchar, exposure
to the scanf and printf functions, C statements, conditional executing
using if, else. Optionally switch
and break statements may be mentioned, Concepts of loops, example of loops
in C using for, while and do-while.
Optionally continue may be mentioned, One dimensional arrays and
example of iterative programs using arrays, 2-d arrays. Use in matrix computations, Concept of
Sub-programming, functions.
Example of functions.
Argument passing mainly for the simple variables, Pointers,
relationship between arrays and pointers.
Argument passing using pointers.
Array of pointers. Passing
arrays as arguments, Strings and C string library, Structures and
Unions. Defining C structures,
passing strings as arguments.
Programming examples, File I/O.
use of fopen, fscanf and fprintf routines.
Text /
Reference Books
1.
Fundamentals of
Computers by V. Raja Raman.
2.
C Language by Brian
Gottfried by Schaum Series.
3.
Introduction to
Computers by Leon & Leon.
SECOND SEMESTER EXAMINATION
L T P S Credits Hours
16 6
10 0 27 32
BA-128 FOUNDATION
COURSE IN PHYSICS - II
1.
Electricity and magnetism: Electric fields, Gauss' Law, its integral and differential
form, applications. Lorentz force, fields due to moving charges, the magnetic
field, Ampere's law, motion of a charged particle in an electric and magnetic
field, magnetic and electrostatic focussing, Hall effect, determination of e/m
by cathode ray tube, positive rays, Thomson's parabolic method, Isotopes, Mass
spectrographs (Aston and Bainbridge), Electron microscope, Cyclotron and
Betatron.
2.
Quantum statistics: The
Statistical distributions: Maxwell Boltzmann, Bose-Einstein and Fermi-Dirac
statistics, their comparisons, Fermions and Bosons. Applications: Molecular
speed and energies in an ideal gas. The Black-body spectrum and failure of
calssical statistics to give the correct explanation - the application of
Bose-Eientein statistics to the Black-body radiation spectrum, Fermi-Dirac
distribution to free electron theory, electron specific heats, Fermi energy and
average energy - its significance.
3.
Band theory of solids: Origin
of energy bands in solids, motion of electrons in a periodic potential- The
Kronig-Penny model. Brillouin zones, effective mass Metals. semi-metals.
semi-conductors and insulators and their energy band structure. Extrinsic and
intrinsic semiconductors, doping - Fermi energy for doped and undoped
semiconductors, the p-n junction (energy band diagrams with Fermi energy), the
unbiased diode, forward and revesrse biased dides- its characteristics, tunnel
diode, zener diode, photo-diode, LED, the photo-voltaic cell, the transistor,
its characteristics, common base, common emmiter, common collector, load line,
relation between a
and b.
4.
Superconductivity: Introduction
to superconductivity, the Meissner effect, Type I and II superconductors, the
Josephson effect, flux quantization, Cooper pais, the BCS theory (qualitative),
properties and applications of superconductors.
Text / Reference Books
1.
Modern Physics by A.
Beiser.
2.
Optics by A.K. Ghetak
3.
Modern Physics by
Hallday & Resvik.
4.
Introduction to
Physical Optics by Jenkin & White
BA 108 MATHEMATICS
- II
1. Linear Algebra
: Linear Independence
and dependence of vectors, Systems of linear equations- consistency and
inconsistency. Gauss elimination method, rank of a matrix, Bilinear, Quadratic,
Hermitian, Skew-Hermitian Forms, Eigenvalues and Eigenvectors of a matrix,
diagonalization of a matrix, Cayley-Hamilton Theorem (without proof).
2.
Ordinary Differential Equations : Formation of ODEs, definition of order, degree and
solutions. ODEs of first order :
Method of separation of variables, homogeneous and nonhomogeneous equations,
exactness and integrating factors, linear equations and Bernoulli
equations. General linear ODEs of nth
order : solutions of homogenous and nonhomogenous equations, operator method,
method of undetermined coefficients and variation of parameters. Solutions of simple simultaneous ODEs.
Power series method of solution of DE, Legendres Equation, Legendres
Polynomials, Bessels equation, Bessels function.
3.
Complex Variables
: Curves and Regions in the Complex Plane, Complex Functions, Limits,
Derivative, Analytic Function, Cauchy-Riemann Equations, Laplaces Equation,
Linear Fractional Transformations, Conformal Mapping, Complex Line Integral,
Cauchys Integral Theorem, Cauchys Integral Formula, Derivatives of Analytic
Function, Power Series, Taylor Series, Laurent Series, Methods for obtaining
Power Series, Analyticity at Infinity, Zeroes, Singularities, Residues, Residue
Theorem, Evaluation of Real Integrals.
4.
Probability :
Definition of Sample Space, Event, Event Space, Conditional Probability,
Additive and Multiplicative law of Probability, Bayes Law theorem, Application
based on these results.
Text / Reference Books
1.
N.M. Kapoor
Differential Equations Pitamber Pub. Co.
2.
Schaum Outline Series
Differential Equations Mc. Graw Hill.
3.
Schaum Outline Series
Complex Variables Mc. Graw Hill.
4.
Schaum Outline Series
Linear Algebra Mc. Graw Hill.
5.
Schaum Outline Series
Probability Mc. Graw Hill.
BA-132 FOUNDATION
COURSE IN ORGANIC CHEMISTRY - II
1.
Reactive intermediates-
Generation, structure and general
reactions of carbocations, carbanions, free radicals and carbenes (singlet and
triplet). Wagner-Meerwein
rearrangement, Electrophiles and nucleophiles, concepts of acids and
bases. Bronsted theory, Lewis theory 1
and Person's Classification (HSAB), Carbon acids (active methyiene groups),
super acids, Correlation of structure with acidity and basicity. Hyperconjugation : concept and consequences.
Field effect, Resonance effect - Resonance energy and its significance,
(vertical and empirical resonance energy), Strains in acyclic compounds.
2.
IUPAC Nomenclature : Systematic IUPAC
nomenclature of different classes of compounds including aromatic, bicyclic,
and spiro compounds and polyfunctional compounds.
3.
Stereochemistry
: Classification of stereomers,
diastereoiners, Separation of enantiomers.
Absolute configuration (R and S), Projection formulae. Stereochemistry of compounds containing two
asymmetric C-atoms. Elements of
symmetry - centre, plane, axis of symmetry, Stereochemistry of biphenyls and
spiro compounds, Conformations :
Conformations around a C-C bond in acyclic compounds, Structure of
cycloalkanes, different. Strain in
cyclic compounds, Cyclohexane conformations, Stereochemistry of disubstituted
cyclohexanes. Geometrical isomerism-
Concept, E and Z nomenclature, Stereoselective and specific Reactions. Introduction to asymmetric synthesis. Bonds weaker than covalent bond : Hydrogen bonding - nature, types,
stability and effects, van der Waals
forces, Electron-donor acceptor complexes.
Inclusion compounds.
4.
pP - dP bonding in organic compounds, ylids (S and P), Wittig reaction.
5.
Tautomerism
: Cationotropy and anionotropy, Prototropic shifts in different systems,
ring-chain tautomerism and valence tautomerism, Cope rearrangement.
6.
Alkanes : Methods of preparation, Source-petroleum and
coal in brief, Cracking and reforming.
7.
Alkenes : Methods of
preparation. Reactions: Hydrogenation,
oxidation, hydroxylation, addition- Markownikoff rule with explanation and
peroxide effect. Dienes - types of
dienes and their characteristic reactions, effect of conjugation on stability
and reactivity, Diels-alder reaction in detail with its stereochemistry. Polymerisation of olefinic compounds, Use
and mechanism of Ziegler-Natta catalysts, Hydroboration reaction, Claisen
rearrangement.
Text / Reference Books
1.
Modern Organic
Chemistry by D.R. Boyed.
2.
Organic Chemistry by
I.L. Finar.
3.
Organic Chemistry
Reaction Mechanism by Jerry March.
BA 122 LIFE SCIENCES - II
1.
Coordination and control: Plant
movements (Tactic, Tropic, Nastic), plant growth substances (Auxins,
Cytokinins, Gibberellins, ABA, Ethylene),
phytochrome and effect of light on plant development, vernalisation and
flowering. Nervous system, parts of the nervous system, sensory receptors,
structure and function of receptors, Endocrine system, role of hormones in
growth and development of humans.
2.
Homeostasis: Control
system in biology, control of blood glucose level, temperature regulation in
endothermic animals, the liver and its importance.
3.
Mendalian Analysis: Experiments
of Mendel, Simple mendalian genetics in humans, in agriculture, Variants and
genetic dissection.
4.
Asexual Reproduction: Apomixis,
and other means of natural vegetative reproduction (Bulb, corm, rhizome,
stolon, runner, tuber, tap roots, tillers), advantages and disadvantages of
natural asexual reproduction.
Artificial propagation - cutting, grafting, budding, layering, micropropagaion
through tissue culture, advantages and disadvantages of micropropagation.
5.
Sexual reproduction:
Life cycle of flowering plants, the parts of a flower (Dicot and monocot),
microsporogenesis, in-vitro pollen culture, microgametogenesis, isolation of
sperms, palynology, scope of palynology, development of ovule, types of ovule,
megasporogenesis, megagametogenesis, embryosac, function of different cells of
embryosac, pollination types of pollination, pollen-pistil interaction, self
incompatibility, fertilization, double fertilization, post fertilization
changes in ovule and embryo, seed formation, structure of seed and its
importance.
Review of sexual reproduction in vertebrates, human
intervention in reproduction.
6.
Continuity of life: Chromosome,
cell cycle, mitosis and meiosis, techniques to study mitosis and meiosis.
7.
Heredity and Variation: Mendel's
work, chromosomal basis of inheritance, modified dihybrid ratios, gene
interaction, linkage, gene mapping, sex determination, cytoplasmic inheritance,
variation and mutation.
8.
Economically Important Plants : Classification systems, Important families (Fabaceae,
Poaceae, Malvaceae, Cucurbitceae, Crucifereae, Leguminoseae), Cereals (wheat,
rice maize), Beverages (tea, coffee, cocoa), Fibers (jute, linen, cotton), wood
(pines, cedar, teak, sisham), rubber (para rubber), spices (turmeric, black
pepper, cloves, coriander), medicinal plants (Ephedra, Taxus, Cinchona, Fox
glove, Belladonna, Rauvolfia, Neem, Hemp.)
Text
/ Reference Books:
BT124
LABORATORY TECHNIQUES IN BIOTECHNOLOGY
1.
pH, Buffers: Principles and theory, pH
meters.
2.
Colorimetry & Spectroscopy: Basic
principles, nature of electromagnetic radiation, Beer-Lambert laws,
colorimetric methods & instruments, principles of spectroscopy, types of
spectra-absorbance, emission, fluorescence and action spectra, single and
double beam spectrophotometers, densitometers, flame photometer, fluorimeters,
circular dichorism & their applications.
3.
Cell separation and flow cytometry, magnetic
beads, elutriator.
4.
Microscopy: Basic principles,
instrumentation, sample preparation for optical, phasecontrast, interference,
polarization, inverted fluorescence, confocal & electron microscopes &
their applications.
5.
Microtomy: Principles & types, sample
preparation & sectioning parameters.
6.
Centrifugation: Principles & types
simple & differential, ultracentrifugation-preparative & analytical.
7.
Chromatography: Principles, methodology
and applications of chromatography using paper, thin layer, column (gel
filtration, ion exchange, affinity), gas, HPCL, FPCL etc.
8.
Electrophoresis: Principles and types of
electrophoresis and their applications for proteins, nucleic acids, including
gradient gel and pulse-filed gel electrophoresis; gel matrices: polyacrylamide,
agarose etc. critical parameters for optimum separation and resolution, two
dimensional electrophoresis (IEF).
9.
Radioisotope methods and tracer techniques in
biology: Basic principles of radioactivity, properties &
handling of radioisotopes in biology & medicine, radiation units, Geiger
Muller & scintillation counters, autoradiography, radionucleide imaging, CT
scan.
10.
Biophysical Techniques: X-ray
crystallography Nuclear Magnetic Resonance (NMR) spectra, magnetic Resonance
Imaging (MRI), lasers in biology and medicine, Mass spectrometry.
Text
/ Reference Books:
1. Biochemical
Calculations by Irwin H. Segel, John Wiley & Sons (2nd Edition),
1975
IT 120 ELECTRICAL SCIENCE
1.
Properties of Conductors and Insulators : Basic laws of Electrical Engineering, Temperature Resistance
Coefficients
2.
D. C. Circuits : Network
theorems and applications, Division of Current, Potentiometer, Circuit
parameters, Energy and power, Superposition, Thevenin and Reciprocity theorems,
Star Delta Formations
3.
Alternating Currents : Peak,
Average and RMS values for alternating currents, Power and Power factor ,
Resistance, Inductance and Capacitance, Resonance, Q Factor.
4.
Measuring Instruments : Electromagnetism,
Moving Coli and Moving Iron, Instruments, Construction Instruments, Attraction
and Repulsion type, Permanent Magnet and Eletrodynamics, Dynamometer type.
5.
D. C. Generators & Motors : Principle of operation of Generators & Motors, Speed
Control of shunt motors, Flux control, Rheostatic control, voltage control,
Speed control of series motors.
6.
A. C. Generators & Motors : Principle of operation, Revolving Magnetic field, Squirrel
cage and phase wound rotor, Starting of Induction motors, Direct on line and
Star Delta starters, Synchronous machines.
7.
Transformers: Construction,
Regulation and efficiency calculations, Open and short circuit tests.
Text / Reference Books
1.
Electrical
Engineering Fundamentals by Vincent DEL TURU.
HUGHES, Electrical Technology.
THIRD SEMESTER EXAMINATION
L T P S Credits Hours
13
5 14 0
26 32
BT-201 MICROBIOLOGY
1.
Microbes in our lives:
Types of microorganisms. Brief history
of microbiology. Microbes & human
warfare. Microbes & human disease.
2.
Observing Microorganisms through a Microscope: Light, electron, scanned-probe microscopy. Simple, differential and special stains.
3.
Functional Anatomy of Prokaryotic and Eukaryotic Cells: Size, shape, and arrangement of bacterial cells. Structures external to cell wall, structures
internal to cell wall. Microbial
Metabolism.
4.
Catabolic & anabolic reactions: enzymes, energy production and carbohydrate
metabolism. Lipid & protein
catabolism, bacterial identification and photosynthesis. Energy production mechanism, metabolic diversity
& pathways of energy use.
Integration of metabolism.
5.
Microbial Growth:
Growth requirements, culture media, obtaining pure cultures and preservation of
cultures, growth of bacterial cultures, Control
of Microbial Growth, Action of microbial control agents, physical and
chemical methods of microbial control.
6.
A survey of the Microbial World: Classification of microorganism and methods of classifying
and identification of microorganism.
7.
The prokaryotes groups: domain
bacteria, proteobacteria, nonproteobacteria Gram-ve and Gram+ve bacteria. Bacterial diversity.
8.
Fungi, lichens, algae, protozoa, helminthes, arthropods as
vectors.
9.
Viruses: viral
structures, isolation, cultivation and identification of viruses, viral
multiplication.
10.
Principles of disease and epidemiology: Mechanism of
microbial pathogenicity.
11.
Antimicrobial Drugs. History, spectrum and action of
antimicrobial drugs. Tests to guide
chemotherapy and effectiveness of chemotherapeutic agents.
12.
Applied & Industrial Microbiology: Industrial fermentation, primary and secondary metabolites,
Role of microorganisms in the production of industrial chemicals and
pharmaceuticals, Microbes as alternative energy sources and as industrial
products.
Text / Reference Books:
1.
Microbiology: An Introduction: Tortora, Funke & Case.
7th edition, 2001
2.
Microbiology: Davis, Dulbecco, Eisen and Ginsburg.
3.
Introduction to
Microbiology: Ross
4.
General
Microbiology: Stainier, Adelberq and
Ingraham.
BA-203 BIOENERGETICS
- I
1.
Biochemical Evolution: Chemogeny,
Biogeny, and Evolution of Chromosome Organization and Genetic Regulatory
Mechanisms, Time factors in evolution, Evolution of Enzyme Systems.
2.
Amino Acids and Peptides: Structure,
Function, Methods of Characterization, Separation Techniques based on their
structure and properties, Clinical Significance, Biosynthesis.
3.
Carbohydrates: Mono
and Polysaccharide, Classification, Structure, Function, Separation and
Characterization Techniques, Clinical significance, Biosynthesis.
4.
Lipids: Classification,
Structure, Function, Separation and Characterization Techniques, Clinical
Significance.
5.
Nucleic Acids: Nucleic
Acids and Polynucleotides, Classification, Structure, Function, Separation and
Characterization Techniques, Clinical Significance.
6.
Vitamins and Micro and Macro Nutrients: classification, Structure, Function, Separation and
Characterization Techniques, Clinical Significance.
7.
Biochemical Energetics: Energy
Yielding and Energy Requiring Reactions, Calculations of Equilibrium
Concentrations, Oxidation-Reduction Reactions, Metabolism and ATP Yield.
Photosynthetic Phosphorylation, Active Transport, Second Law of Thermodynamics,
Enthalpy and Entropy, Activation Energy.
8.
Spectrophotometry and other Optical methods: Spectrophotometry, Flurometry, Optical Rotation -
Polarimetry, Photochemistry, and Quantum efficiency.
Text / Reference Books
1.
Biochemistry
by Lubert Stryer. W. H. Freeman & Company, NY
2. Biochemistry by Lehninger. McMillan publishers
3. Biochemistry by Zubey. Wm. C. Brown publishers
BT- 205 CELL
BIOLOGY
1.
The Cell:
a macromolecular assembly, cellular compartmentalization, organeller
architecture
2.
The Nucleus: Chromosomal
DNA and its Packaging, The Global Structure of Chromosomes, Chromosome
Replication, RNA Synthesis and RNA Processing, The Organization and Evolution
of the Nuclear Genome.
3.
Cytoskeleton: The
Nature of the Cytoskeleton, Intermediate Filaments, Microtubules, Cilia and
Centrioles, Actin Filaments, Actin-binding Proteins, Muscle.
4.
Cell Junctions, Cell Adhesion, and the Extracellular Matrix
: Cell Junctions,
Cell-Cell Adhesion, The Extracellular Matrix of Animals, Extracellular Matrix
Receptors on Animal Cells- the Integrins, The Plant Cell Wall
5.
Membrane Structure, Transport of Molecules and Membrane
Excitability: The Lipid Bilayer,
Membrane Proteins, Principles of Membrane Transport, Carrier Proteins and
Active Membrane Transport, Ion channels and Electrical Properties of Membranes
6.
Protein Sorting and Vesicular Trafficking in the Cell: The Compartmentalization of Higher Cells, The Transport of
Molecules into and out of the Nucleus, The Transport of Proteins into
Mitochondria and Chloroplasts, Peroxisomes, The endoplasmic reticulum., Transport from the ER through the
Golgi Apparatus, Transport from the Trans Golgi Network to Lysosomes, Transport
from the Plasma Membrane via Endosome: Endocytosis, The Molecular Mechanisms of
Vesicular Transport and the Maintenance of Compartmental Diversity.
7.
Cell Signaling: General
Principles of Cell Signaling, Signaling via G-Protein-linked Cell-Surface
Receptors, Signaling via Enzyme-linked Cell-Surface Receptors, Kinase
Receptors, Structural Features of Trans-membrane Receptors, Hormone Receptor
Interaction, Two-component signaling, Second messengers.
8.
Cell Cycle and Division: The
General Strategy of the cell Cycle, The
Mechanics of Cell Division, The Early Embryonic Cell Cycle, Cell- Cycle control
in Yeasts and Multicellular Animals.
9.
Cancer: Cancer as a
Microevolutionary Process, Tumor cells, Proto-oncogenes and viral oncogenes,
Tumor suppessor genes.
Text / Reference Books
1.
Molecular Biology of Cell by Albert et.al. John
Wiley & Sons
2.
The Cell by Cooper.
ASM Press
3.
Cell and Molecular
Biology by Karp. John Wiley & Sons
BT-209 GENETICS
1.
Genetics and Organism: Genetics
and human affairs, Genetics and Biology, Genes and Environment, Techniques of
genetic analysis.
2.
Chromosome Theory of Inheritance: The chromosome theory of heredity, Sex chromosomes, Sex
linkage, The parallel behaviour of autosomal genes and chromosomes.
3.
Mendelian Analysis: Mendels laws of inheritance, Interaction of genes, Variations on dominance, Multiple
alleles, Lethal alleles, Several genes affecting the same character, Penetrance
and expressivity,
4.
Linkage: Basic
eukaryotic chromosome mapping, The discovery of linkage, Recombination linkage
symbolism, Linkage of genes on X chromosomes, Linkage maps, Three point
testcross, Interference, Calculating recombinant frequencies from selfed
dihybrids, examples of linkage maps, The X2 test mitotic segregation
in humans.
5.
Fine Structure of Genes: The
concept of promoter, Coding sequence, Terminator, Induction of gene for
expression.
6.
Recombination in Bacteria and Viruses: Conjugation recombination and mapping the E. coli chromosomes, Transformation,
Transduction, Chromosome mapping.
7.
Biochemical Genetics: Inborn
errors of metabolism, one gene one enzyme hypothesis, one gene one protein,
one gene one polypeptide, colinearity
8.
The Extranuclear Genome: The
concept of extranuclear genome in higher plants and animals, Overview of
mitrechondrial genome, Chloroplast genome.
9.
Population Genetics: Darwins
revolution, Variation and its modulation, The effect of sexual reproduction on
variation, The sources of variation, Selection quantitative genetics
10.
Principles of Plant Breeding: Objectives, Selfing and crossing techniques, Male sterility,
Incompatability, Hybrid vigour
11.
Human Genome Project:
Genetic diseases in humans, Genetics and society.
References
1.
In Introduction to
genetic analysis, Griffiths, Miller, Suzuki, Lewontin and
2.
Gelbart, Freeman and
Company.
3.
Genetics, A.V.S.S. Sambamurty,
Narosa Publishing House.
4.
Concepts of Genetics,
Klug & Cummings, Prientice Hall.
5.
Molecular Cloning,
Moniatisetal, Cold Spring Harbor Laboratory.
BT-211 BIOSTATISTICS
1. Presentation of Data: Frequency
distribution, graphical presentation of data by histogram, frequency curve and
cumulative frequency curves.
2. Measure of Location and Dispersion:
Mean, Medium, Mode and their simple properties (without derivation) and
calculation of median by graphs: range, mean deviation, Standard deviation, Coefficient
of variation.
3. Probability and Distribution: Random
distributions, events-exhaustive, mutually exclusive and equally likely,
definition of probability (with simple exercises), definition of binomial,
Poisson and normal distributions and their inter-relations, Simple properties
of the above distributions (without derivation).
4. Correlation and Regression: Bivariate
data simple correlation and regression coefficients and their relation,
Limits of correlation coefficient, Effect of change of origin and scale on
correlation coefficient, Linear regression and equations of line of regression,
Association and independence of attributes.
5. Sampling: Concept of population and
sample, Random sample, Methods of taking a simple random sample.
6. Tests of Significance: Sampling
distribution of mean and standard error, Large sample tests (test for an
assumed mean and equality of two population means with known S.D.); small
sample tests (t-test for an assumed mean and equality of means of two populations
when sample observations are independent, Paired and unpaired t-test for
correlation and regression coefficients, T-test for comparison of variances of
two populations, Chi-square test for independence of attributes, Goodness of
fit and homogeneity of samples.
7. Experimental Designs: Principles of
experimental designs, Completely randomized, Randomized block and latin square
designs, Simple factorial experiments of 22, 23, 24 and 32 types, Confounding
in factorial experiments (mathematical derivations not required); Analysis of
variance (ANOVA) and its use in the analysis of RBD.
Reference:
1.
Statistical methods in biology by Norman T.J. Bailey (3rd
Edition), Cambridge University Press (1995).
CT-211 CHEMICAL ENGINEERING - I
1.
Chemical engineering
discipline, structure and practice.
2.
Stoichiometry and
chemical equations. Units, dimensions
and conversions. Phase rule, Henrys
law, Raults law and their applications to gas-liquid and vapor-liquid systems.
3.
Material balance for
non-reacting and reacting systems, recycle and by pass.
4.
Heats of solution,
mixing and reactions. Types of energy
and first law of thermodynamics.
5.
Energy balance for
non-reacting and reacting systems.
Calculation of flame temperature and adiabatic reaction temperature.
6.
Properties of fluids
& fluid statics. Mechanical energy
balance.
7.
Flow of
incompressible fluids: laminar and turbulent flows, velocity distribution in
pipes, pressure drop in pipes and fittings.
8.
Stokes law and its
applications.
9.
Flow in packed beds.
10.
Flow measurement:
Orifice & Venturi meter. Pumps and their characteristics.
Text / Reference
Books
1.
Unit Operations of
Chemical Engineering, McCabe W.L., Smith J.C. and Harriott P., McGraw Hill
International Edition, Singapore, 5th Ed., 1993.
2.
Chemical Engineering,
Vol. 1, Coulson J.M. and Richardson J.F., Butterworth Heinemann, Oxford 6th
Ed., 1999
3.
Fluid Mechanics,
Douglas J.F., Gasiorek J.M., Swaffield J.A., Addison-Wesley Longman, 3rd
Ed., 1995.
4.
Basic Principles of
Calculations in Chemical Engineering, Himmelblau D.M., Prentice Hall, 6th
Ed., 1999.
5.
Elementary Prnciples
of Chemical Processes, Felder R.M. and Rousseau R.W., John Wiley & sons,
Inc., 3rd Ed., 2000.
FOURTH SEMESTER EXAMINATION
L T P S Credits Hours
14 5 13 0 26 32
BT-202 IMMUNOLOGY
1.
Introduction
to Immunology: Properties of immune
response, Innate and acquired immunity, active and passive immunity.
2.
Cells &
Tissues of Immune System: Lymphocytes,
Classes of lymphocytes, antigen presenting cells, NK Cells, Mast Cells,
Dendritic Cell, Organs of the Immune System, Bone marrow, Thymus, Lymph node,
Spleen, CALT, MALT.
3.
Molecular Immunology:
- Molecular structure of antibody, Classification, Isotypes, Synthesis assembly
and expression of immunoglobulin molecules, Nature of antigens, function and
diversity, Generation of anti-body diversity.
4.
Antigens:
Different characteristics of antigens, mitogens, Hapten, Immunogen, Adjuvants.
5.
MHC: Discovery of MHC complex, Role of MHC, Structure of MHC
molecule, Binding of peptides to MHC molecules, MHC restriction.
6.
Effecter
Mechanism of Immune Response:
Cytokines, T- cell receptors, cell activation, complement system, antigen
processing and presentation, regulation of immune response.
7.
Immunological Techniques:-
antigen- antibody reactions, Immuno diffusion, immunoelectro- phoresis,
ELISA, RIA, fluorescence activated cell
sorter,
8.
Applied Immunology:- Immune system in health and disease,
autoimmunity, hyper-sensitivity, tumor immunity, tissue and organ transplant,
Synthetic vaccines.
9.
Hybridoma technology:
- Fusion of myeloma cells with lymphocytes, production of monoclonal antibodies
and their application.
Text / Reference books:
1.
Kuby- Immunology (4th
Edition) by R. A. Goldsby, T.J. Kindt, B.A. Osborne.
2.
Essentials of
Immunology (6th Edition): Ivan Riot- Blakswell Scientific
Publications, Oxford, 1988.
3.
Fundamentals of
Immunology: Paul W.E. (Eds.) Raven
Press, New York, 1988.
4.
Antibodies A
laboratory Manual: Harlow and David Lane (1988), Cold spring harbor
laboratory.
BT-204 MOLECULAR
BIOLOGY
1.
Structure and properties of nucleic acids: Models of DNA structure; RNA structure; Physical, Chemical,
Spectroscopic Nuclear & Organelle genomes,
2.
Genome Complexity: L
C value paradox, cot analysis, Repetitive DNA, Satellite DNA, Pseudo genes,
Synteny.
3.
Chromosome organization:
Histones, Non-histones, Nucleosome, Chromatin, Chromosome structure in
prokaryotes & eukaryotes,
4.
Gene organization:
Split Genes, Overlapping genes, Transposons & Retrotransposons, Gene
clusters,
5.
DNA-Protein interaction:
DNA- binding motifs, Methods of studying DNA binding proteins,
6.
DNA Replication: Models
of DNA replication, Enzymology of DNA replication, The Replication process,
Initiation, Elongation & Termination of replication; Telomeres.
7.
Transcription and mRNA processing: Components of transcriptional machinery in prokaryotes and
eukaryotes; Initiation, Elongation & Termination of transcription; Capping,
Polyadenylation, Splicing, mRNA stability.
8.
Translation: The
Genetic code; tRNA & aminoacyl synthetases, Ribosomes, Translation process,
Initiation, Elongation & termination of transcription; Capping,
Polyadenylation, Splicing, mRNA stability.
9.
Regulation of gene expression: General aspects of regulation prokaryotes & eukaryotes;
The operm model, lac & trp operons; DNA methylation; Tissue-sp. &
developmental stage sp. Expression of genes.
12. Gene Mutation:
Somatic vs germinal mutation,
Mutant types, Selective Systems, Induction of mutation, Chromosomal mutations,
Changes in chromosome structure mutation and cancer, Mutagens in genetic
dissection, Mutation breedings, Molecular basis of gene mutations, Repair
defects and human diseases, Recombination, Transposable genetic elements.
10. Molecular
evolution: DNA based phylogenetic
trees and their applications.
Text
/ Reference Books:
1.
Gene VII
by B. Lewin.
2.
Essentials
of molecular Biology, Malacinski and Freifelder Jones and Bartlelt Publishers.
3.
Genomes,
T. A. Brown, John Wiley and Sons PTE Ltd.
4.
Cell and
molecular Biology, Concepts and experiments Gerald Karp, John Wiley and Sons.
5.
The Cell
- A molecular approach, Gm Cooper Asm Press.
BT-206 ENZYME TECHNOLOGY
2.
Structural Features of Enzymes: Chemical nature of Enzymes: amino acids, the building
blocks of protein, Levels of protein Structure: Primary, secondary, tertiary
and quaternary structure.
3.
Specificity of Enzymes:
Types of specificity, the Koshland induced fit hypothesis, Strain or
transition state stabilization hypothesis.
4.
Enzyme Catalysis and Kinetics: Factors affecting the rate of chemical reactions, kinetics
of uncatalyzed chemical reactions, kinetics of enzyme-catalyzed reaction, methods
for investigating the kinetics of enzyme-catalyzed reactions, nature of enzyme
catalysis, inhibition of enzyme activity.
5.
The Investigation of Active Site Structure and Chemical
nature of Enzyme Catalysis: The identification of binding sites and catalytic
site, three dimensional structure of active site, mechanism of catalysis,
mechanism of reaction catalyzed by enzyme without cofactors, metal-activated
enzyme and metalloenzyme, coenzymes in enzyme catalyzed reactions.
6.
Immobilization of Enzymes:
Concept, methods of immobilization, Kinetics of immobilized enzymes, effect of
solute partition and diffusion on kinetics of immobilized enzymes, use of
immobilized enzymes, bioreactors using immobilized enzyme.
7.
Industrial uses of enzymes:
Industrial enzymes: Sales value of industrial enzymes, traditional
(non-recombinant) sources of industrial enzymes, The impact of genetic
engineering on enzyme production, Engineered enzymes, Extremophiles: hyperthermophiles,
enzymes from hyperthermophiles, enzymes from additional extremophiles, enzymes
in organic solvent
8.
Industrial
enzymes: proteases and carbohydrases:
Proteolytic enzymes: Carbohydrases, Lignocellulose degrading enzymes, Pectin
and pectic enzymes.
9.
Additional
industrial enzymes: Lipases,
Penicillin acylase, Amino acylase and amino acid production, cyclodextrins and
cyclodextrin glycosyl transferase, enzymes in animal nutrition,
Oxidoreductases, Enzymes in molecular biology.
10.
Enzyme Engineering:
Prediction of enzyme structure, design and construction of novel enzymes.
Text / Reference Books:
1.
Enzymes by Palmer
(2001): Horwood Publishing Series.
2.
Fundamentals
of Enzymology by Price and Stevens (2002): Oxford University Press.
3.
Enzyme
Technology by Helmut uhling (1998): John Wiley
4.
Introduction
to Proteins Structure by Branden and Tooze (1998): Garland Publishing Group.
BA - 208 BIOENERGETICS II
1.
Catabolism and the Generation of Chemical Energy
2.
Metabolic Strategies: General
Principles of Intermediary Metabolism, Regulation of Pathways, Strategies for
Pathway Analysis
3.
Glycolysis, Gluconeogenesis, and the Pentose Phosphate
Pathway : Glycolysis,
Gluconeogenesis, Regulation of glycolysis and Gluconeogenesis, The pentose
Phosphate Pathway
4.
The Tricarboxylic Acid Cycle: Discovery of the TCA Cycle, Steps in the TCA Cycle,
Stereochemical Aspects of TCA Cycle Reactions, ATP Stoichiometry of the TCA
Cycle, Thermodynamics of the TCA Cycle, The Amphibolic Nature of the TCA Cycle,
The Glyoxylate Cycle, Oxidation of other Substrates by the TCA Cycle,
Regulation of TCA Cycle Activity
5.
Electron Transport and Oxidative Phosphorylation: The Mitochondria Electron - Transport Chain, Oxidative
Phosphorylation, Transport of Substrates, Pi, ADP and ATP into and out of
Mitochondria, Electron Transport and ATP Synthesis in Bacteria,
6.
Photosynthesis and other Processes Involving Light : Photosynthesis,
Other Biochemical Processes Involving Light
7.
Metabolism of Fatty Acids : Fatty Acid Degradation, Biosynthesis of Saturated Fatty
Acids, Regulation of Fatty Acid Metabolism
Text / Reference Books
1.
Biochemistry
by Lubert Stryer. W. H. Freeman & Company, NY
- Biochemistry by Lehninger.
McMillan publishers
- Biochemistry by Zubey. Wm. C.
Brown publishers
CT-212 CHEMICAL ENGINEERING-II
1.
Mixing: types of
agitators, flow patterns and power consumption.
2.
Steady state conduction:
Fouriers law, concept of resistance to heat transfer, critical insulation
thickness, conduction with heat generation.
3.
Convection:
Film theory and concept of heat transfer coefficient. Heat transfer in laminar and turbulent flows.
4.
Heat Exchanger:
sizing of shell & tube heat exchangers. Heat transfer in agitated vessels.
5.
Boiling & Condensation: heat transfer to boiling liquids and from condensing
vapours.
6.
Fundamentals of mass transfer: moleuclar diffusion in fluids and solids, concept of mass
transfer coefficient. Equilirbium
stage, multistage and contious contactors with applications to gas absoption,
calculation of NTU, HTU and number of stages.
7.
Psychrometric chart
and its applications.
Text / Reference Books
1.
Transport
Processes and Unit Operations, Geankoplis C.J., Prentice Hall of India, 3rd,
1999.
2.
Heat Transfer,
Holman J.P., McGraw Hill, New York, 8th Ed 1997.
3.
Unit
Operations of Chemical Engineering, McCabe W.L., Smith J.C. and Harriott P.
McGraw Hill International edition, Singapore, 5th Ed., 1993.
4.
Chemical
Engineering, Vol. I and II, Coulson J.M. and Richardson J.F. Butterworth
Heinemann, Oxford, 6th Ed., 1999.
FIFTH SEMESTER
EXAMINATION
L T P S Credits Hours
12 5 12
0 25 30
BT-301 PLANT TISSUE CULTURE
1.
History: Important
events in the history of plant tissue culture.
2.
Laboratory Requirements and General Techniques: Introduction, requirements, techniques.
3.
Cellular Totepotency: Introduction,
cyto-differentiation, orgemogenic differentiation, loss of morphogenic
potential in long-term cultures, practical applications of cellular totipotency
4.
Tissue Culture Media: Introduction,
media constituents, media selection, media preparation
5.
Cell and Suspension Culture: Introduction, isolation of single cells, suspension
cultures, culture of single cells, plant cell reactors, applications of cell
culture.
6.
Proloplast Culture: Proloplast
isolation, culture and regeneration.
7.
Somatic Embryogenesis:
Introduction, some examples of formatic embryogenesis, factors affecting
somatic embryogenesis, induction and development, maturation
8.
Haploid Production: Introduction,
techniques, factor affecting androgenesis, ontogeny of androgenic haploids,
plant regeneration from pollen embryos, gynogeresis, haploid production through
disport hybridization idiptridization to raise homozygous diploids,
applications, limitations.
9.
Triploid Production: Introduction,
callusing, histology and cytology of cells, organogenesis, applications of
endosperm culture.
10. Embryo
Culture: Introduction,
techniques, culture requirements role of the suspensor in embryo culture,
precocious germination, morphogenesis in the culture of seeds with partially
differentiated embryos, micronugical experiments, embryo and seed culture of
parasitic angiosperms, morphogenic potential of the embryo callus, practial
applications.
11. In-vitro
pollination and fertilization: Introduction,
terminology, in vitro pollination, in vitro fertilization, applications.
12. Micropropagation:
Introduction, techniques,
applications, production of pathogen free plants
13. Production of
secondary metabolites: Introduction,
strategies used to optimize product yield, commercial aspects
14. Germplasm
Storage: Introduction,
long-term storages, short or medium term storage
Text
/ Reference Books:
- Experiments
in Plant Tissue Culture by John H. Dodds & Lorin W. Robert.
2.
Plant tissue Culture
: Theory and Practice by S.S. Bhojwani
and M.K. Razdan (1996) Elsevier, Amsterdam.
3.
An Introduction to
Plant Biotechnology by H C Chawla Oxford and IBH 2002
BT-303 MICROBIAL
PROCESS ENGINEERING
1.
Bio Process Development:
An interdisciplinary challenge, major classes of products
- Introduction to Engineering
calculation, presentations and analysis of data: Physical variables, dimensions,
units, errors in data and calculations, testing mathematical models,
process flow diagram
- Material Balances: Thermodynamics Law of conservation
of mass, types of material balance products, electron balances, biomass
yield, theoretical oxygen demand., problems
- Energy Balances: Basic concepts, General Energy
balance equations, Enthalpy calculations, Enthalpy changes in non-reactive
processes, Types of energy balance calculations, Types of heat reactions,
problems
- Unsteady state material and energy
balances: Material
balance equation for CSTR, Energy balance equations, solving
differential equations, solving
mass balances, solving energy balances, problems
- Fluid flow and mixing: Classification of fluids, Reynolds
number, Momentum transfer, Non Newtonian fluids, Two-Parameter models, rheological properties of
fermentation broths, mixing, power requirements for mixing, scale-up of
mixing systems, role of shear in stirred fermentors, problems
- Heat transfer: Equipments, mechanism of heat
transfer, conduction, heat transfer between fluids, design equation for heat transfer systems, applications
of design equations, problems
- Mass transfer: Molecular diffusion, role of
diffusion in bioprocessing, film theory, convective mass transfer, oxygen
uptake and transfer in cell
cultures, kLa determination, problems.
- Homogeneous reactions: Basic reaction theory, calculation
of reaction rates, general reaction kinetics for biological systems,
yields in cell culture, cell growth kinetics, production kinetics, kinetics
of cell death, problems
- Heterogeneous reactions:Concentration gradients and
reaction rates in solid catalysts, internal mass transfer and reaction,
the Thiele modulous and effectiveness factor, external mass transfer,
problems
- Reactor Engineering: Bioreactor configurations,
practical considerations for bioreactor construction, monitoring and
control of bioreactors, ideal reactor operations, batch operation of a
mixed reactor, case study of penicillin production.
Text / Reference
Books:
1.
Bioprocess Engineering
- Basic concepts by M. L. Schuler & F. Kargi, Entice Hall 1992.
2.
Bioprocess
Engineering Principles by Pauline M. Doran, Academic Press 1995.
3.
Fermentation &
Biochemical Engineering Hand Book
(1983), Principles, Process Design and Equipment. HC Vogel, Noyes.
4.
Principal of
Microbe & Cell Cultivation
(1975), SJ Prit, Blackwell Scientific co.).
5.
Bioprocess
Computations in Biotechnology (Vol. 1) TK Ghose, Ellis howard Ltd.
BT-305 Animal Biotechnology
1. Introduction to Animal Tissue Culture: Background,
Advantages, Limitations, Application, Culture Environment, Cell Adhesion, Cell
Proliferation, Differentiation.
2.
Design, Layout and Equipment: Planning,
Construction, Layout, Essential Equipments, Aseptic Technique, Objectives,
Elements, Sterile Handling, Safety, Risk Assessment, General Safety, Fire,
Radiation, Biohazards
3.
Media: Physicochemical Properties,
Balanced Salt Solutions, Complete Media, Serum, Serum-Free Media, Disadvantages
of Serum, Advantages of Serum-Free media
4. Primary Culture: Isolation
of Tissue, Steps involved in primary cell culture, Cell Lines, Nomenclature,
Subculture and Propagation, Immortalization of cell lines, Cell line
designations, Routine maintenance
5. Characterization & Quantitation of
Cell Line: Need for characterization, Morphology, Chromosome Analysis,
DNA Content, RNA and Protein, Enzyme Activity, Antigenic Markers,
Transformation, Immortalization, Aberrant Growth Control, Tumorigenicity, Cell
counting, DNA content, Protein, Rates of Synthesis, Cell Proliferation, Plating
Efficiency, Labeling Index, Generation Time.
6.
Contamination: Source of contamination,
Type of microbial contamination, Monitoring, Eradication of Contamination,
Cross-Contamination
7.
Cryopreservation: Need of Cryopreservation,
Preservation, Cell banks, Transporting cells
8.
Cytotoxicity: Introduction, In vitro
limitations, Nature of assay, Viability assay, Survival assay, Microtitration
assay, Transformation assay
9. Transgenic Animals: Methodology,
Embryonic Stem Cell method, Microinjection method, Retroviral vector method,
Applications of transgenic animals
10. Gene Therapy: Ex-vivo
gene therapy, In vivo gene therapy, Viral gene delivery system, Retrovirus
vector system, Adenovirus vector system, Adeno-Associated virus vector system,
Herpex simplex virus vector system, Non-viral gene delivery system, Prodrug
activation therapy, Nucleic acid therapeutic agents
11. In Vitro Fertilization and Embryo
Transfer: Composition of IVF media, Steps involved in IVF,
Fertilization by means of micro insemination, PZD, ICSI, SUZI, MESA
Text / Reference
Books:
1. Animal
Cell Culture by John R.W. Masters
Oxford
University Press
2. Introduction
to Cell and Tissue Culture by Jennie P. Mather and Penelope E. Roberts
Plenum
Press, New York and London
3. Molecular
Biotechnology: Primrose.
4. Animal Cell Biotechnology: R.E. Spier
and J.B. Griffiths (1988), Academic press.
BT-307 RECOMBINANT
DNA TECHNOLOGY & APPLICATION
1.
Basic concepts in
genetic engineering
2.
Tools of
Genetic Engineering: Cloning
vehicles, Restriction enzymes, Modifying enzymes, DNA ligase, Polymerase etc.
3.
Cloning
Vectors: Plasmids, Lambda
phage, Phagemids, Cosmids, Artificial chromosomes (BACs, YACs), Shuttle
vectors, virus based vectors.
4.
Methods of
gene transfer: Transformation,
transduction, Particle gun, Electroporation, liposome mediated, microinjection,
Agrobacterium mediated gene transfer.
5.
Preparation
and application of molecular probes:
DNA probes, RNA probes, Radioactive labeling, Non radioactive labeling, use of
molecular probes, DNA fingerprinting.
6.
Analysis and
expression of cloned gene in host cells:
Expression vectors, Restriction enzyme analysis, Southern blotting, Northern
blotting, Western blotting, In-situ hybridization. Colony and plaque hybridization, Factors affecting expression of
cloned genes, Reporter genes, Fusion proteins.
7.
Gene libraries
- cDNA synthesis, Genomic DNA
libraries, Amplification of gene libraries, Identifying the products of cDNA
clones.
8.
Isolation,
Sequencing and synthesis of gene:
Different methods of gene isolation, Techniques of DNA sequencing, Artificial
DNA synthesis.
9.
Polymerase
Chain reaction (PCR): Basic
principles, modifications, applications.
10. Modifying Genes:
Site-directed mutagenesis, Insertion & Deletion Mutagenesis.
Text /
Reference Books:
1.
From Genes to Clones
by Winnacker. PANIMA
2.
Molecular
Biotechnology by Pasternack and Glick.
3.
From Genes to
Genomes: Concepts & Applications of DNA Technology by J.W. Dale & M.V.
Schartz.
4.
Gene Cloning &
DNA Analysis: An Introduction (4th edition) by T.A. Brown.
5.
Molecular Cloning by
Sambrook, et al.
6.
Principles of Gene
Cloning by Old and Primrose.
BT- 206 DEVELOPMENT
BIOLOGY
1.
History & Basic Concepts: The origins of developmental biology, Concepts in
development Developmental signals in cell division & differentiation,
Role of gene expression in development, Identifying developmental genes, Cell
commitment & differentiation, Determination & induction of cell fate,
Concept of morphogen & positional information.
2.
An Introduction to Model Systems: Model vertebrate organisms: X. laevis, Chicken, Mouse, Zebrafish, Model invertebrate organisms:
D. melanogaster, C. elegans, Model
plant: A. thaliana
3.
Germ Cells & Sex, Genotypic
& phenotypic sex-determiantion in mammals, D. melanogaster and C. elegans,
Structure & Formation of germ cells, Fertilization
4.
Patterning the Vertebrate Body Plan : Axes & Germ Layers, Setting up the body axes, The
origin & specification of the germ layers, The Mesoderm & Early Nervous
System, Somite formation & Patterning, Role of the organizer region &
neural induction
5.
Development of the Drosophila: Body Plan,
Specification of body axes & role of maternal genes, Polarization of body
axes during oogenesis, Patterning of early embryo & role of zygotic genes,
Segmentation & role of pair-rule genes, Compartments & role of segment
polarity genes, Selector & Homeotic genes
6.
Development of Nematodes & Cellular Slime Molds: Developmental axes determination in C. elegans, Cell-fate
specification in C. elegans, Larval development in C. elegans, Vulva
development in C. elegans, Patterning of the slug in slime mold, Cell
differentiation in slime mold, Aggregation
7.
Morphogenesis: Kinds
of cleavage & blastulation, Types of tissue movement in gastrulation,
Gastrulation in amphibians & mammals, Neural tube formation & neural
crest migration
8.
Cell Differentiation & Organogenesis: Models of cell differentiation, Insect imaginal disc & wing development
9.
Molting & Metamorphosis: Amphibian metamorphosis, Insect metamorphosis
10.
Plant Development: Pattern
development in early embryogenesis of angiosperms, Floral development
Text /Reference Book:
1. Developmental Biology, by Scott F.
Gilbert (1997), Sinauer Associates, Inc.
SIXTH SEMESTER EXAMINATION
L T P S Credits Hours
2 5 12 0 25 30
BT-302 BIOINFORMATICS
1.
The Internet and Biologist: Internet basics, FTP, Gopher, World wide web.
2.
The Gen Bank Sequence Database: Introduction, Primary & Secondary databse, Format vs content:
computer vs humans, GenBank Flat File dissection, GCG, ACDEB.
3.
Structure Databases:
Introduction to structures, PDB, MMDB, Structure file formats, Visualizing
structural information, Database structure viewers.
4.
Information Retrieval from Biological Databases: Retrieving database entries, Integrated information
retrieval: The entrez system, sequence databases beyond NCBI, Medical Databases
5.
The NCBI Database:
Introduction, SeqIDS, Bioseq: Sequences, Bioseqsets: Collections of sequences,
Seq. Annot: Annotating the sequence, Seqdiscr: Describing the sequence
6.
Sequence Alignment and Database Searching: Introduction, Evolutionary basis of sequence alignment,
Optimal alignment methods, Substitution scores & gap penalties, Statistical
significance of alignments, Database similarity searching, FASTA, BLAST, Low
complexity regions, Repetitive elements
7.
Multiple Sequence Alignment: Progressive alignment methods, Motifs and patterns, Hocks,
MOST, Probe, Presentation methods, Abscript
8.
Phylogenetic Analysis:
Elements of phylogenetic models, data analysis: Alignment, substitution model
building, tree building and tree evaluation, building methods, searching for
trees, hooting trees, Evaluating trees and data, phylogenetic software Some
simple practical consideration
9.
Predictive Methods Using Nucleotide Sequence: Framework, marking repetitive DNA, Database search, Codon
bias detection, Detecting function sites in the DM, Integrated gene passing,
Finding tRMA genes
10.
Predictive methods Using Protein Sequences: Protein identity based on composition, Propsearch, Physical
properties based on sequences, secondary structure and folding classes, Sspread
sopma, Specialized structures of features, Tertiary structure
11.
Genome Mapping:
Different types of maps: physical, genetical, etc. Synteny, Human genome
project, Application of genome mapping, Chromosome maps.
12.
Submitting DNA Sequences to the Databses: Introduction, Where to submit, What to submit, How to
submit on the world wide web, How to submit with sequin.
Reference:
1. Bioinformatics:
A practical guide to the analysis of genes
and proteins A.D. Baxevanis and B.F.F. Ouellette (Eds). 2002 John Wiley
and Sons.
2. Bioinformatics: Sequence and Genome Analysis by D.W. Mount,
2001, Cold Spring Harbor Laboratory Press.
BT-304 FOOD
BIOTECHNOLOGY
1.
Historical Background:
History of Microorganisms in food, Historical Developments.
2.
Taxonomy, role and
significance of microorganisms in foods.
Intrinsic and Extrinsic Parameters of Foods that affect microbial
growth.
3.
Microorganisms in
fresh meats and poultry, processed meats, seafoods, fermented and fermented
dairy products and miscellaneous food products.
4.
Starter cultures,
cheeses, beer, wine and distilled spirits, SCP, medical foods, probiotics and
health benefits of fermented milk and foods products.
5.
Brewing, malting, mashing, hops, primary & secondary
fermentation: Biotechnological improvements: catabolic repression, High gravity
brewing, B-glucan problem, getting rid of diacetyl. Beer, wine and distilled spirits.
6.
Nutritional
boosts and flavor enhancers:
Emerging processing and preservation technologies for milk and dairy products.
7.
Microbiological Examination of surfaces, Air Sampling, Metabolically Injured Organisms,
Enumeration and Detection of Food-borne Organisms. Bioassay and related Methods
8.
Food Preservation,
Food Preservation Using Irradiation, Characteristics of Radiations of Interest
in Food Preservation. Principles Underlying the Destruction of Microorganisms
by Irradiation, Processing of Foods for Irradiation, Application of Radiation,
Radappertization, Radicidation, and Radurization of Foods Legal Status of Food
Irradiation, Effect of Irradiation of Food constituents.
9.
Storage Stability Food
Preservation with Low Temperatures, Food Preservation with High Temperatures,
Preservation of Foods by Drying, Indicator and Food-borne Pathogens, Other
Proven and Suspected Food-borne Pathogens.
10. Psychrotrophs, Thermophiles and Radiation-resistant Microorganisms,
Characteristics and Growth of Thermophilic Microorganisms, Nature of Radiation
Resistance in Microorganisms. Rheology
of Food Production.
11. Consumer
perspective and future of food biotechnology.
Text / Reference Books:
- Modern
Food Micro-Biology by James M. Jay, (2000), 6th edition, An
Aspen Publication, Maryland, USA.
- Food
Microbiology: Fundamentals and frontiers by M.P. Doyle, L.R. Beuchat and
Thoma J. Montville, (2001), 2nd edition, ASM press, USA.
- Food
Science and Food Biotechnology by G.F.G. Lopez & G.V.B. Canovas
(2003), CRC Press, Florida, USA.
BT-306 PLANT
BIOTECHNOLOGY
1. Introduction Definition, Classical vs modern approach
2. Production of
disease free plants explant, shoot
tip culture, shoot tip grafting, viricidtel compounds
3. Micropropagation
Basic technique, Automation in
the area scope as an commercial venture.
4. Tissue Culture
as some of Genetic Variability Somaclonal
and gametoclonal variation, Selection, Sources and causes of variation,
Application in crop improvement.
5. Protoplast
Related Techniques Protoplast,
Isolation, Culture and fusion, Selection of hybrid cells, regenration of hybrid
plants, somatic hybridization and cybridization, Applications in crop
improvement.
6. Plant as
Biofactories Concept, Production
of Chemicals, Pigments, Perfume, Flavors, Insecticides, anticancer agents and
other important compounds.
7. Transformation
Techniques Physical methods, Agrobacterium, Mediated transformation
8. Transgenics Basic concept and essential steps of the process, Some
examples of transgenic plants, Use of suitable promoters, Gene silencing and measures
to overcome it, Commercial aspects of the technology.
9. Nitrogen
Fixation Basic concepts, nif
genes and their regulation, potential scope in crop improvement
10.
Transformation of organelles: Methods and success, advantages of organller transformation.
11.
Molecular Markers Concept,
SNPs, RAPD, RFLP, ISSR, STMS, role in crop improvement and genome mapping.
Text / Reference Books
1.
Plant Tissue
Culture: Applications and
Limitations. S.S. Bhojwani (1990), Elsevier, Amsterdam.
2.
Micropropagation:
P.C. Debergh and R.H. Zimmerman (1990),
Kluwer Academic Publ. Dordrecht.
3.
Transgenic plants
Lindsey and Jones
4.
Plants, genes &
crop improvement, Crispeels ASPB, 2002
5.
Agricultural
Biotechnology A. Altman.
BT-308 DOWN STREAM PROCESSING
1.
Biomolecules of Commercial importance Ethanol, citric acid, lysine, steroids, penicillin, dextran,
trehalose, subtilisin, chymosin, vitamin B12, hepatitis B vaccine, insulin,
erythropoietin, monoclonal antibodies.
2.
Techniques and Instrumentation Filtration, centrifugation,
aqueous two phase system, ion exchange chromatography, gel permeation
chromatography, affinity chromatography, spectrometry, automation, bioassay,
automated sequencers, mass spectrometry, ORD,CD
3.
Proteins from microbes, plants and animal sources by
classical and modern biotechnology Recombinant
versus non-recombinant proteins, Microorganisms as source of proteins, Protein
production in genetically engineered microorganism such as E. coli, yeast and
fungi, Proteins from plants, Production of heterologous proteins in plants,
Animal tissues as protein source, production in transgenic animals, animal cell
culture, insect cell culture.
4.
Large scale protein purification Production of factor VIII, t-PA, hepatitis B, Asparaginase,
insulin, interferon alfa, glucose oxidase, horse radish peroxidase, Alfa
amylase, subtilising, lipase, casein, whey protein concentrate.
5.
A General Study of Various Classes of Commercial Proteins Blood products, vaccines, therapeutic antibodies and enzyme
hormones and growth factors, interferon, interleukins, industrial enzymes,
non-catalytic industrial proteins.
6.
A General Study of Commercial Products other than Proteins Bulk organics (ethanol), Biomass (Bakers Yeast), Organic
acids (Citric Acid), Amino Acids (L-Lysine), Microbial Transformations
(Steroids), Antibiotics (Penicillin), Extra Cellular Polysaccharides (Xanthan
Gum), Nucleotides (5-GMP), Vitamins
(B12), Pigments (Shikonin)
Reference:
1.
Protein: Biochemistry
and Biotechnology by Gary Walsh (2002 John Wiley & Sons Ltd.)
2.
Process Biotechnology
Fundamentals by S.N. Mukhopadhyay (2001). Viva Books Private Limited.
BT-310 BIOSENSORS
1. Introduction
to Biosensors: Concepts and applications.
2. Biosensors
for personal diabetes management.
3. Microfabricated Sensors and the
Commercial Development of the I- stat Point-of-Care system.
4. Noninvasive Biosensors in Clinical
Analysis.
5. Surface
Plasmon Resonance.
6. Biosensors
based on Evanescent Waves.
7. Applications of Biosensor-based
instruments to the bioprocess industry.
8. Application of Biosensors to
environmental samples.
9. Introduction to Biochips and their
application in modern sciences.
Text
/ Reference Books:
1.
Commercial Biosensor: Grahm Ramsay, John Wiley & Son,
INC. (1998)
BT-312 Biochemical Engineering & Biotechnology
1. Introduction
to Biotechnology: What is biotechnology, An interdisciplinary pursuit,
A three-component central core, Product safety, Public perception of
biotechnology, Biotechnology and the developing world.
2. Amino Acids, Peptides and Proteins: Structure,
Function, Methods of Characterization, Separation Techniques based on their
structure and properties.
3. Nucleic Acids: Nucleic Acids and
Polynucleotides, Classification, Structure, Function, Separation and
Characterization Techniques.
4. Biochemical Energetics: Energy
Yielding and Energy Requiring Reactions, Calculations of Equilibrium
Concentrations, Oxidation-Reduction Reactions, Metabolism and ATP Yield.
Photosynthetic Phosphorylation, Active Transport, Second Law of Thermodynamics,
Enthalpy and Entropy, Activation Energy.
5. Metabolic Strategies: General
Principles of Intermediary Metabolism, Regulation of Pathways, Strategies for
Pathway Analysis.
6. Bioprocess/fermentation
technology: Bioreactor, Scale-up, Media design, Technology for
microbial, mammalian and plant cell culture, Downstream processing.
7. Enzyme
Technology: Nature, Application, Genetic engineering & protein
engineering, Immobilised enzymes and Technology of enzyme production.
8. Biopharmaceuticals:
Introduction to genetic engineering, Antibiotics, Therapeutic proteins,
Vaccines & monoclonal antibodies, Gene therapy.
9. Food and
beverage technology: Introduction, Fermentation, Food processing,
Sweeteners, Food wastes, Rapid diagnostics, Public acceptance & safety.
10. Agricultural
and forestry Biotechnology: Introduction, Plant biotechnology, Forestry,
Biological control, Animal biotechnology, Diagnostics in agriculture,
Bioremediation.
11. IPR,
Safety, Social, moral and ethical aspects of Biotechnology
Text / Reference Books
1.
Biochemistry
by Lubert Stryer. W. H. Freeman & Company, NY
- Biochemistry by Lehninger.
McMillan publishers
- Biochemistry
by Zubey. Wm. C. Brown publishers
- Biotechnology, John E. Smith
- Bioprocess Engineering Principles, Pauline M.
Doran
SEVENTH SEMESTER EXAMINATION
L T P S Credits Hours
13 5 12
0 25 30
BT-401 STEM
CELLS IN HEALTH CARE
1.
Introduction:
Stem Cell Biology, Fate Mapping of Stem Cells
2.
Stem Cell
Pattern: Differentiated Parental
DNA Chain Causes Stem Cell Pattern of Cell-type Switching in Schizosaccharomyces pombe
3.
On Equivalence
Groups and the Notch/LIN-12
Communication System,
4.
Cell Cycle Control, Checkpoints,
and Stem Cell Biology, Senescence of Dividing Somatic Cells
5.
The Drosophila Ovary:
An In Vivo Stem Cell System
6.
Male Germ-line
Stem Cells
7.
Primordial
Germ Cells as Stem Cells,
Embryonic Stem Cells, Embryonal Carcinoma Cells as Embryonic Stem Cells,
Trophoblast Stem Cells
8.
Hematopoietic Stem Cells:
Repopulating Patterns of Primitive Hematopoietic Stem Cells, Molecular
Diversification and Developmental Interrelationships, Hematopoietic Stem Cells:
Lymphopoiesis and the Problem of Commitment Versus Plasticity, Hemangioblast
9.
Mesenchymal
Stem Cells of Human Adult Bone
Marrow
10. Stem Cells and Neurogenesis
11. Epidermal Stem
Cells: Liver Stem Cells,
Pancreatic Stem Cells, Stem Cells in the Epithelium of the Small Intestine and
Colon
Text / Reference Books:
1.
Developmental
Biology, 6th Edition, Scott F. Gilbert
2.
Hematology, William
J. Williams, Ernest Beutler, Allan JU. Erslev, Marshall A. Lichtman
3.
Molecular Biology of
the Cell, 3rd Edition, Bruce Alberts, Dennis Bray, Julian Lewis,
Martin Raff, Keith Roberts, James D. Watson
4.
Stem Cell Biology by
Marshak, 2001, Cold Spring Harbar Symposium Pulblication.
BT-403 ENVIRONMENTAL BIOTECHNOLOGY
1.
Introduction to Environment: Concept of ecology and ecosystem, environmental pollution
(Water, soil and air) noise and thermal pollution, their sources and effects.
2.
Sewage and waste water treatments
anaerobic and
aerobic treatment, conventional and advanced treatment technology, methanogenesis, methanogenic, acetogenic,
and fermentative bacteria- technical
process and conditions, emerging biotechnological processes in waste -
water treatment.
3.
Solid waste management:
landfills, composting, earthworm treatment, recycling and processing of organic
residues.
4.
Biodegradation of xenobiotic compounds, organisms involved in degradation of chlorinated
hydrocarbons, substituted simple
aromatic compounds, polyaromatic hydrocarbons, pesticides, surfactants and
microbial treatment of oil pollution.
5.
Bioremediation and Biorestoration: Reforestation through
micropropagation, development of stress tolerant plants, use of mycorrhizae in
reforestation, use of microbes for improving soil fertility, reforestation of
soils contaminated with heavy metals.
6.
Microbial leaching and mining: Extraction of
metals from ores; Recovery of metals from solutions; Microbes in petroleum
extraction; Microbial desulfurization of coal.
7.
Environmental Biotechnology in Agriculture: Biofertilizers and microbial inoculants, biopesticide,
bioinsecticides, bioherbicides
8.
Biofuel: Plant
derived fuels, Energy crops, Biogas, Bioethanol, biohydrogen
9.
Environmental genetics:
degradative plasmids, release of genetically engineered microbes in
environment.
10.
Biosafety and Biethics in Biotechnology
11.
Environmental laws and policies
Text / Reference Books:
1.
Environmental
Biotechnology by Alan Scragg (1999); Longman.
2.
An Introduction to
Environmental Biotechnology by Milton Wainwright (1999): Kluwer Academic Press.
BT-405 PROTEIN
BIOTECHNOLOGY
1. Protein Structure: Introduction, Overview of protein
structure, Higher level structure, Protein post-translational modification,
Protein stability and folding.
1.
Protein
Sources: Introduction,
Microorganisms as sources of proteins, Proteins from plants, Animal tissue as a
protein source, Direct chemical synthesis, Conclusion.
2.
Protein
Purification and Characterization:
Introduction, Initial recovery of proteins, Removal of whole cells and cell
debrisConcentration and primary purification, Column chromatography, Protein
inactivation and stabilization, Protein characterization.
3.
Large-Scale
Protein Purification: Some general
principles, Therapeutic protein production: some special issues, Range and
medical significance of impurities potentially present in protein-based
therapeutic products, Labelling and packing of finished products.
4.
Therapeutic
Proteins: Introduction, Blood
products, Haemophilia A and B, Anticoagulants, Thrombolytic agents, Additional
blood-related products, Vaccine technology, Vaccines for AIDS.
5.
Therapeutic
Antibodies and Enzymes: Introduction,
Antibodies for in vivo application, Therapeutic enzymes.
6.
Hormones and
Growth Factors used Therapeutically:
Introduction, Insulin, Glucagon, Gonadotrophins, Growth hormone,
Erythropoietin, Other growth factors, Thyrotrophin, Corticotrophin, Prolactin,
Peptide Regulatory Factors.
7.
Interferons,
Interleukins and Additional Regulatory Factors: Regulatory factors; cytokines versus hormones, Interferons,
Interleukins, Tumour necrosis factors, Colony-stimulating factors, Cytokine
toxicity.
8.
Proteins Used
for Analytical Purposes: Introduction,
Enzymes as diagnostic/analytical reagents, Biosensors, Antibodies as analytical
reagents.
9.
Non-catalytic
Industrial Proteins: Introduction,
Functional properties of proteins, Milk and milk proteins, Animal and microbial
proteins, Sweet and taste modifying proteins.
Text
/ Reference Books:
1.
Proteins: Biochemistry and Biotechnology by Gary Walsh.
(2002): John Wiley & Sons Ltd.
2.
Fundamentals of Protein Biotechnology: Edited by Stanley
Stein (1990): Marcel Dekker, Inc.
BT-407 MARKETING
& MANAGEMENT OF BIOTECHNOLOGY
1.
Why there
is a need to commercialize biotechnology.
Discovery, market needs, development process, success rates and costs
etc.
2.
Creating
and marketing the image of the biotechnology Company. Art of negotiation & effective communication.
3.
Role of
venture capitalism, business plan, selection of CEO and personnel, real estate
for a biotech start-up.
4.
How to
portray management and role of a biotechnology manager, technology
decision-making, and resource decision-making etc., Product marketing
decision.
5.
Role of
Research & development University-industry technology transfer
arrangements, how and why a biotech company can benefit.
6.
Positioning,
power and importance of positioning of a company name and product, Workable
marketing and the strength of distribution.
7.
Effective
advertising and marketing. Opportunities
international, marketing and lessons to be learned.
8.
Indian
and foreign prospective of biotechnology, and current challenges for the
biotechnology based products.
Text / Reference Book:
1.
Positioning
by All Rise and Jack Trout (1986), Warner Books.
2. Biotechnology: The science & the business by V. Moser
& R.E. Cape (1999) Harwood.
3. Latest review articles and papers on the subject.
HS-409 WRITING
SKILLS FOR TECHNICAL PURPOSES
1.
Making Technical Text Readable: Logic and Organizational Patterns; Language and Visual
display.
2.
Gathering Date:
Interviewing; Using the Library/Internet; Listing Reference Material.
3.
Paper and Report Writing:
Organising a Paper; Writing the Discussion or Body of an Article; Writing the
exit; Writing the Lead.
4.
Writing Specific Documents: Letters and Memos; Job Applications, Cover letters and
Resume.
5.
Designing and Writing for Electronic Media: Using Internet as a Writing Tool; Designing and Writing for
Multi-media; Writing and Designing for World Wide Web.
6.
Oral Presentations:
Listening ans Speaking Skills
Text
/ Reference Books:
1.
How to Write &
Present Technical Information, 3rd Edition, Charles H. Sides,
Cambridge University Press. 1999.
2.
Garffey, Mary Ellen
Business Communication, Cincinnati: South-Western College Publishing, 2000
3.
Parley E Stevens and
Daniel G Riardaw. Technical Report Writing Today N Delhi AITBS, 1998.
EIGHTH SEMESTER EXAMINATION
L T P S Credits Hours
9
3 18 0
30 30
BT-402 INDUSTRIAL BIOTECHNOLOGY
1.
Introduction, Objectives and Scope; Characteristic and
comparison of bioprocessing with chemical processing.
2.
Substrates for bioconversion processes and design of media
3.
Isolation, preservation and improvement of industrial
microorganisms, Cell culture techniques and aseptic transfers
4.
Metabolic basis for product formation. Production of secondary
metabolites-penicillin, tetracycline etc
5.
Process technology for the production of cell biomass and
some primary metabolites, e.g. ethanol, acetone-butanol, citric acid, dextran
and amino acids.
6.
Microbial production of industrial enzymes-glucose
isomerase, cellulase & lipases.
7. Applications of bioconversion,
transformation of steroids and sterols. Transformation of non-steroidal
compounds, antibiotics and pesticides.
7.
Bioenergy-fuel from biomass, production and economics of
biofuels.
8. Metal
recovery and microbial desulfurization of coal.
Text / Reference Books:
1.
Comprehensive Biotechnology Vol. 1- 4: M.Y. Young (Eds.), Pergamon Press.
2.
Biotechnology: A Text Book of Industrial Microbiology: T.D.
Brock, Smaeur Associates, 1990.
3.
Industrial Microbiology: L.E. Casida, Willey Eastern Ltd.,
1989.
4.
Industrial Microbiology:
Prescott & Dunn, CBS Publishers, 1987.
5.
Bioprocess Technology- fundamentals and applications, S O
Enfors & L Hagstrom
(1992),
RIT, Stockholm.
6.
Biotechnology, Economic
& Social Aspects: E.J. Dasilva, C Rutledge & A Sasson, Cambridge Univ. Press,
Cambridge.
7. Biotechnology - a handbook of
industrial microbiology: W. Crueger and A. Crueger.
8. Microbial
Biotechnology: Channarayaappa, University press, Hyderabd, 2003
BT-404 INTELLECTUAL PROPERTY RIGHTS IN
BOTECHNOLOGY
1.
WTO: As an
international agency controlling trade among nations. WTO with refernce to biotechnological affairs, TRIPs.
2.
General Introduction:
Patent claims, the
legal decision making process, ownership of tangible and intellectual
property.
3.
Basic Requirements of Patentability Patentable subject matter, novelty and the public domain,
non obviousness
4.
Special issues in Biotechnology Patents Disclosure requirements, Collaborative research, Competitive
research, plant
5.
Plant biotechnology Indian
patents and Foreign patents, Plant variety protection act, The strategy of
protecting plants.
6.
Patent Litigation Substatritive
aspects of patent litigation, Procedural aspects of patent litigation,
different Doctrines
7.
Recent Developments
in Patent System and Patentability of biotechnological inventions.
8.
IPR issues in Indian Context Role of patent in pharmaceutical industry, computer related
innovations
9.
Case studies Rice, Haldi, neem, etc. and challenges
ahead
Text / Reference Books:
1.
The law and strategy
of Biotechnological patents by Sibley. Butterworth publications.
2.
Intellecutla property
rights Ganguli Tat McGrawhill
3.
Intellectual property
right Wattal Oxford Publishing House.
BT-406 MOLECULAR AND CELLULAR
DIAGNOSTICS
1.
General Clinical Laboratory Techniques &
Procedure: Chemical & Related substrates, volumetric analysis,
Balancing & Weighing, Concept of solute & solvent, Units of measurement
2. Specimen Collection & Processing: Specimen
collection (Blood, urine, spinal fluid, saliva synovial fluid, Amniotic fluid),
Preservation, transportation
3. Selection & Interpretation of
Lab. Procedure: Classification of
BIAS, Sensitivity and specificity, Receiver Operator Characteristics,
Interpretation a test
4. Quality Management: Fundamentals
of total quality management, Element of QAP, External quality assessment and
proficiency testing programme.
5. Clinical Enzymology: Principle
of diagnostic enzymology, Liver, cardiac and skeletal enzyme, Digestive enzyme,
Miscellaneous enzyme
6. General Function Tests: Liver
function test, Cardiac Function Test, Renal Function Test, Thyroid Function
test, Reproductive endocrime function test
7.
Immunodiagnostics:
Introduction, Antigen-Antibody Reactions, Conjugation Techniques,
Antibody Production, Enzymes and Signal Amplification Systems, Separation and
Solid-Phase Systems, Case studies related to bacterial, viral and parasitic
infections.
8.
Product
Development: Immunoassay Classification and Commercial Technologies,
Assay Development, Evaluation, and Validation, Reagent Formulations and Shelf
Life Evaluation, Data Analysis, Documentation, Registration, and Diagnostics
Start-Ups.
9.
DNA based diagnostics: PCR, RFLP, SSCP, Microarrays, FISH, In-situ
hybridization, Case studies related to bacterial, viral and parasitic
infections.
10.
Cell based diagnostics: Antibody markers, CD Markers, FACS, HLA typing,
Bioassays.
11.
Biosensors:
Concepts and applications, Biosensors for personal diabetes management,
Noninvasive Biosensors in Clinical Analysis, Introduction to Biochips and their
application in modern Sciences, Introduction to Nanotechnology.
Text / Reference
Books:
1.
Tietz Textbook of
Clinical Chemistry, Carl A. Burtis, Edward R. Ashwood, Harcourt Brace &
Company Aisa Pvt. Ltd.
2.
Commercial
Biosensors: Graham Ramsay, John Wiley & Son, INC. (1998).
3.
Essentials of
Diagnostic Microbiology, Lisa Anne Shimeld.
4.
Diagnostic
Microbiology, Balley & Scotts.
5.
Tietz Text
book of Clinical Biochemistry, Burtis & Ashwood.
6.
The Science of
Laboratory Diagnosis, Crocker Burnett.
BT-408 BIOSAFETY & BIOETHICS
- Biotechnology and Society: Introduction to science,
technology and society, biotechnology and social responsibility, public
acceptance issues in biotechnology, issues of access, ownership, monopoly,
traditional knowledge, biodiversity, benefit sharing, environmental
sustainability, public vs. private funding, biotechnology in international
relations, globalisation and development divide.
- Bioethics: Legality, morality and ethics,
the principles of bioethics: autonomy, human rights, beneficence, privacy,
justice, equity etc.
- Biotechnology and Bioethics: The expanding scope of ethics
from biomedical practice to biotechnology, ethical conflicts in
biotechnology - interference with nature, fear of unknown, unequal
distribution of risks and benefits of biotechnology, bioethics vs.
business ethics, ethical dimensions of IPR, technology transfer and other
global biotech issues.
- Biosafety concepts and issues: Rational vs. subjective
perceptions of risks and benefits, relationship between risk, hazard,
exposure and safeguards, biotechnology and biosafety concerns at the level
of individuals, institutions, society, region, country and the world.
- Biosafety in the laboratory
institution:
Laboratory associated infections and other hazards, assessment of
biological hazards and levels of biosafety, prudent biosafety practices in
the laboratory/ institution
- Biosafety regulations in the handling of recombinant DNA
processes and products in institutions and industries, biosafety
assessment procedures in India and abroad
- Biotechnology and food safety: The GM-food debate and biosafety
assessment procedures for biotech foods & related products, including
transgenic food crops, case studies of relevance.
- Ecological safety assessment of recombinant organisms and
transgenic crops, case studies of relevance (Eg. Bt cotton).
- Biosafety assessment of biotech
pharmaceutical products such
as drugs/vaccines etc.
- International dimensions in
biosafety: Catagena
protocol on biosafety, bioterrorism and convention on biological weapons
Text
/ Reference Books:
1.
Thomas, J.A., Fuch,
R.L. (2002). Biotechnology and Safety Assessment (3rd Ed). Academic
Press.
2.
Fleming, D.A., Hunt,
D.L., (2000). Biological safety Principles and practices (3rd Ed).
ASM Press, Washington.
3.
Biotechnology - A
comprehensive treatise (Vol. 12). Legal economic and ethical dimensions VCH.
4.
Encyclopedia of
Bioethics
M.TECH. SYLLABUS
SCHEME OF EXAMINATION FOR M.TECH. (BIOTECHNOLOGY)
NINTH SEMESTER
EXAMINATION
Code No. L T
Credits
THEORY
PAPERS (Any three)
BT-501 Advances in Agricultural Biotechnology
BT-503 Proteomics
BT-505 Concepts in Biomedical Engineering
BT-507 Genomics
Laboratory
Demonstration & Assistance
Research
Project
|
2
2
2
2
|
1
1
1
1
|
3
3
3
3
5
6
|
|
TOTAL
|
|
|
20
|
TENTH SEMESTER
EXAMINATION
Code No. L T/P
Credits
THEORY
PAPERS (Any three)
BT-502 Computational Biology
BT-504 Biotechnology in Health care
BT-506 Signaling Networks & Cell Cycle Control
BT-508 Metabolic Engineering
Seminars
Project work
|
2
2
2
2
|
1
1
1
1
|
3
3
3
3
5
12
|
|
TOTAL
|
|
|
26
|
ELEVENTH SEMESTER
EXAMINATION
Code No. L T/P
Credits
Project work Report
Presentations & Viva
|
|
|
15
10
|
|
TOTAL
|
|
|
25
|
BT-501 ADVANCES IN AGRICULTURAL BIOTECHNOLOGY
1. Molecular breeding: Concept and
methodology of different types of molecular markers. Role of molecular markers
in crop and farm animal improvement, conservation of biodiversity; Marker
assisted selection; QTL mapping
2. Choloroplast genetic engineering: Methodology,
applications in herbicide resistance, production of biopharmaceuticals, edible
vaccines, foreign gene expression, Limitations
3. Molecular and biochemical basis of
plant disease resistance, signalling pathways, protein kinases, virus induced
gene silencing
4. Molecular basis of plant resistance to
various abiotic stresses like drought, salinity, heavy metals, high
temperature,etc.
5. Genetic engineering of plants:
production of transgenic plants for fungal, bacterial and viral disease
resistance; herbicide resistance, drought and other abiotic stress resistance;
quality parameters: Modification of nitrogen fixing capabilities; gene
pyramiding
6. Transgenic
animals for better farming, whole genome cloning
7. Molecular farming: Use of plants
and animals for production of
neutraceuticals, edible vaccines and other desired products
Text
/ Reference Books:
1.
Agricultural Biotechnology by Arie Altman. Marcel Dekker,
Inc. (2001).
2.
Plants, Genes and Crop Biotechnology (2003) 2nd
Edition by Chrispeels, M.J. & Sadava D.E. American Society of Plant
Biologists, Jones and Bartlett Publishers, USA.
3.
Biochemistry and Molecular Biology of Plants: Edited by
Buchanan B.B., Gruissem W, and Jones RL (2000) American Society of Plant
Biologists, USA.
4.
Various research and review journals like Nature
Biotechnology, Current Opinion, Trends and Annual Reviews.
BT-507 GENOMICS
1. Introduction
Features of prokaryotic, eukaryotic & organellar
genomes, Genome sizes C value paradox, Gene Counting, Structural, Functional
genomics.
2. DNA
Sequencing
Principles of DNA sequencing, Automated DNA sequencing,
Shotgun sequencing contig assembly.
3. Analysis of
sequence data
Analysis
& Annotation ORF, Exon Intron boundaries, Other features of nucleic
acid sequencing, Protein motifs & domains, Databanks, Sequence comparisons.
4. Analysis of
Genetic Variation
Nature of
genetic variation SNP, Methods to study variation RFLP, PCR based methods,
Genome wide comparisons.
5. Comparative
Genomics
Human
genome project, Arabidopsis genome and other genome projects, Synteny,
Genome evolution.
6. Analysis of
Gene Expression
Analysing
transcription Northern blot, RNase protection assay, RT-PCR, Primer extension
analysis, SI-nuclease protection assay, Comparing transcriptomes- subtractive
hybridization, differential display, SAGE, Reporter genes.
7. Proteomics to Study Genes & Genomes
Biochemical
genomics, Yeast two hybrid assay.
8. Recent
advances in Genomics.
Text
/ Reference Books:
1.
Handbook of
Comparative Genomics: Principles and Methodology by Cecilia Saccone, Graziano
Pesole. Wiley-LISS Publication (2003).
2.
Comparative Genomics
by Melody S. Clark. Kluwer Academic Publishers (2001).
3.
Essentials of
Genomics & Bioinformatics C.W. Sensen, Wiley (2003).
4.
Discovering Genomics,
Proteomics & Bioinfo, A.M. Campbell, C.S.H. Press, (2003)
5.
Various research and review journals like Nature
Biotechnology, Current Opinion, Trends and Annual Reviews.
BT-505 TRANSPORT PHENOMENA IN BIOMEDICAL
ENGINEERING
1. Physical Properties of the Body Fluids
and the Cell Membrane:
2. Solute Transport in Biological Systems
3. The Physical and Flow Properties of
Blood
4. Oxygen Transport in Biological Systems
5. Pharmacokinetic Analysis
6. Extracorporeal Devices
7. Tissue Engineering
8. Bioartificial Organs
Text
/ Reference Books:
1.
Basic Transport
Phenomena in Biomedical Engineering by Ronald L. Fournier. Taylor & Francis
(2000).
BT-504 BIOTECHNOLOGY IN HEALTH CARE
1. Therapeutic Aspects of Biomacromolecules:
Introduction, Endogenous pepticdes and proteins, Modification of endogenous
peptides and proteins
2. Immune System: Overview,
Antibody-mediated response, Vaccines, Cell-mediated immune response, Cancer
immunotherapy
3. Oligonucleotides:
Overview, Gene therapy, Antisense therapy, Ribozymes
4. Oligosaccharides: Overview,
Oligosaccharide synthesis, Heparin, Glycoproteins, Polysaccharide bacterial
vaccines, Approaches to carbohydrate-based cancer vaccines
5. Radiological Agents:
Radiosensitizers and Radioprotective agents
6. Cardiovascular Drugs: Myocardial
infarction agents, Endogenous vasoactive peptides, Hematopoietic agents,
Anticoagulants, anthrombotics and hemostatics
7. Chemotherapeutic Agents: Synthetic
antibacterial agents, Lactam antibiotics, Anthelminitic agents, Anthelmintic
agents, Antiamebic agents, Antiviral agents.
8. Endocrine Drugs: Female sex
hormones and analogs, Agents affecting the immune response
9. Drug Targeting Organ-Specific
Strategies: Basic concepts and novel advances, Brain-specific drug
targeting strategies, Pulmonary drug delivery, Cell specific drug delivery.
Text / Reference
Books:
1.
Pharmaceutical Chemistry by Christine M. Bladon. John Wiley
& Sons, Ltd. (2002).
2.
Burgers Medicinal Chemistry and Drug Discovery (5th
edition) by Manfred E. Wolff. A Wiley
& Sons, Inc. (2000).
3.
Drug Targeting Organ-Specific Strategies by Grietje Molema
and Dirk K. F. Meijer. Wiley-VCH. (2002).
BT-506 SIGNALING
NETWORKS & CELL CYCLE CONTROL
1. Protein-Protein
Interactions & Early Events in Signaling: Integrin Signaling, Signal
Transduction to Tyrosin Kinase Growth Factor Receptors, TGF-b Receptors, Wnt, G-Protein, MAPK kinase, Ras Signaling,
2. Methods: Use of chimeric
receptors, Synthetic peptides, Yeast two-hybrid system.
3. Late
Events and Biological Functions of Integrin Signaling: Methods of study of
integrin regulation of MPA kinase cascades, Methods for analysis of
adhesion-dependent cell cycle progression, Integrin modulation of mitogenic
pathways involved, Methods for studying anoikis, Functional analysis of FAK and
associated molecules in cell migration, Integrin signaling in pericellular
matrix assembly,
4. Inside-Out
Signaling by Integrins: Studies of integrin signaling through platelet,
Tracking integrin-mediated adhesion using green fluorescent protein and flow
cytometry, expression cloning of proteins that modify integrin activation,
Regulation of cell contractility by RhoA: stress fiber and focal adhesion
assembly
5. General
Methods for Signaling Studies of Cell Adhesion Molecules: Intra and
Intercellular localization of proteins in tissue in situ, Optical Microscopy,
Wnt signaling.
Text / Reference Books:
1.
Signaling Through
Cell Adhesion Molecules by Jun-Lin Guan. CRC Press (2000).
2.
Signaling Networks
and Cell Cycle Control by J. Silvio Gutkind. Humana Press (2000).
3.
Protein-Protein
Interactions: A Molecular Cloning Manual by Erica Golemis. Cold Spring Harbor
Laboratory Press. (2001).
BT-503 PROTEOMICS
1. Introduction:
The proteome and the Genome, life and death of a protein , protein a
modular structure functional protein famities deducing the protein for Genome
Gene expression, codon Bias and Protein levels.
2. Bridging
genomics and proteomics: The generation of cDNA expression libraries, their
robotic arraying, construction of PCR filters, generation of a nonredundant set
by oligonucleotide fingerprinting, complex hybridizatino on DNA chips, Protein
arrays. Genration of cDNA expression libraries, use of automated technolgoeis
to generate protien arrays and chips, and the application of protein chips in
proteomics, The characterization of the protein complement of a specific cell
type or tissue at a certain time by high-resolution 2-DE, Bridging the current
proteomics and genomic aproaches by mass spectrometry, Future perspectives and
developments.
3. Two-dimensional
polyacrylamide gel electrophoresis for proteome analysis: Brief history of
2-DE, 2-DE with immobilized pH gradients, sample preparation, solubilization,
Reduction, The first dimension: IEF with IPG, Equilibration between dimensions,
The second dimension: SDS-PAGE, resolution, reproducibility of 2-DE, outlook.
4. Detecting
proteins in polyacrylamide gels and on electroblot membranes: Organic dyes
and silver stains, Reverse stains, Colloidal dispersion stains, organic
fluorophore stains, metal chelate stains.
5. Mass
spectrometry-based emthods for protein identification and phosphorylation site
analysis: Background to mass spectrometry, Correlative mass
spectrometric-based identification strategies, De novo sequencing using
mass spectrometric data, Separation methods for phosphorylation site analysis,
present and future challenges and opportunities.
6. Image
analysis of two-dimensional gels: Data acquisition, digital image
procesing, Protein spot detection and quantitation, Gel matching, Data
analysis, data presentation, data bases.
7. Enhancing
high-throughput proteome analysis: the impact of stable isotope labeling:
Sample preparation, two-dimensional gel separation and analysis, Mass
spectrometry: protein identification using MS data, Mass spectrometry: protein
identification using MS/MS data.
8. Applications of Proteome analysis: Mixing
proteomes, protein expression profile,
identification of protein-protein interactions and protein complexes, mapping
proteins complexes, new approaches in proteomics.
9. Recent advances in Proteomics.
Text / Reference Books:
1.
Proteomics: From
Protein Sequence to Function by S.R. Pennington and M.J. Dunn. Viva Books.
Private Limited (2001).
2.
Introduction to
Proteomics by Daniel C. Liebler. Humana Press
BT-505 Concepts
in Biomedical Engineering
1.
Introduction
to Biomedical Engineering
Historical prospective, Role of biomedical engineers
in healthcare system, Professional status of biomedical engineering.
2. Anatomy,
Physiology and Bioelectric Phenomena
Cellular organization, Tissue, Major organ system,
Homeostasis, Neurons, Basic Biophysics tools and relationship, Equivalent circuit
model for the cell membrane, Hodgkin-Huxley model of action potential
3. Modelling of Physiology System
Modelling of human regulatory systems, Modelling of
body dynamics, computer aided modeling. Simulation & analysis of
physiological system, Network representation & biomechanical analysis,
Experimental approach to measurement of electrical, mechanical & chemical
parameter of human body, Biomedical signal processing & Imaging modalities.
4. Compartmental
Analysis
Introduction model postulates, Compartmental
structure, Compartmental analysis, Convective transport between physiologic
compartments.
5. Biomechanics
Principle of mechanics, Bone mechanics, Orthodental
Electromechanical properties, Muscle mechanics, Monitory device.
6. Biomaterial
used in Medicines
Synthetic polymers, Biopolymers, Bioresorbable &
bioerodible materials, metals, thin film grafts & coating, biological
functional materials, Host reaction to biomaterials, Implants, Polymers used in
vascular prosthesis, Contact lense, Rconstructive materials, Cellular response
to foreign materials.
7. Research
Techniques in Biomedical Engineering
Simulation & analysis of physiological system,
Network representation & biomechanical analysis, Experimental approach to
measurement of electrical, mechanical & chemical parameter of human body,
Biomedical signal processing & Imaging modalities.
8. Therapeutic
Equipment
MRI, CT Scan, PET Scanner, Cardiac pacemaker &
defibrillator, Electrical Nerve & muscle stimulator.
Text / Reference Books:
- Introduction
to Biomedical Engineering by John D. Enderle, Susan M. Blanchard, Joseph
Bronzino, Academic Press.
- Biomechanics
Principle & Application by Daniel J. Schneck
- Biomaterial
& Bioengineering Handbook by Donald L. Wise
- Biomedical
Engineering Handbook Vol. 1 by Joseph D Brongino, CRC Press
- Standard
Handbook of Biomedical Engineering & Design by Myer Kutz.